Biochemical characterization of the bi lobe complex in trypanosoma brucei

... Biochemical characterization of the bi- lobe complex in Trypanosoma brucei LADAN GHEIRATMAND A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES ... Structural complexity of the bi- lobe 106 4.4 Characterization of bi- lobe associated proteins reveals extensive connection of bi- lobe to other cellular structures 108 4.5 TbSpef1 stabilizes the ... Biochemical characterization of the bi- lobe revealed a continuous structural network linking the bi- lobe to other singlecopied organelles in Trypanosoma brucei J Biol Chem 2013, Feb 1; 288 (5):3489-99...

Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme potx

... by semiquantitative analysis of transcripts by Northern blotting and also by quantiﬁcation of the enzyme protein by Western blotting Expression of Pf MDH, Pf LDH and Pf MQO in control and drug ... and purity of RNA the gels were stained with ethidium bromide and visualized on a UV transluminator Equal intensity of ribosomal RNA bands in all of the lanes indicated that an equal amount of ... coli and the recombinant enzyme was puriﬁed to homogeneity Functional characterization of the recombinant Pf MDH protein was carried out by analysis of its biochemical characteristics and enzyme...

Báo cáo khoa học: Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB pot

... that the individual domains of the megasynthase may remain folded despite the lack of the KS dimer Minimal polyketide synthase domains in LovB Characterization of the standalone MAT protein The LovB ... in the yield of the soluble protein Pantetheinylation of the ACP in ACPCON didomain was veriﬁed using MALDI-TOF of a tryptic fragment of the puriﬁed protein (data not shown) The LovB CON domain ... phosphopantetheine thiol of the ACP domain The increase in labeling of the ACP-CON didomain may be a result of improved folding of the ACP domain when attached to the larger CON domain, which can...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... seen in the putative T-box DNA-binding domain of t-bet, the STAT protein interaction domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-ﬁnger ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... expression of these genes within humans and mice, of T-bet within Ginbuna crucian carp [27] and STAT6 in mandarin ﬁsh [28] have shown similar widespread expression In the case of STAT6, highest...

Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx

... The Authors Journal compilation ê 2006 FEBS M H Dicko et al Characterization of sorghum peroxidase Table Purication of the major sorghum peroxidase Step apparent mass of 32 kDa (Fig 1B) Together ... health Therefore, knowledge of biochemical properties of the major peroxidase can help on sorghum processing In this study, we have puried and characterized the cationic peroxidase isoenzyme from sorghum ... temperature of SPC4 was between that of HRP C (Tm ẳ 60 C) and the African palm tree peroxidase (Tm ẳ 74 C) [37] In the presence of Ca2+, the Tm of SPC4 is near that of soybean peroxidase, which is one of...

Báo cáo khoa học: Identiﬁcation and biochemical characterization of the Anopheles gambiae 3-hydroxykynurenine transaminase pot

... particular, the biochemical aspects of the 3-HK dependent synthesis of XA in Anopheles gambiae, the most efficient vector of the most deadly malaria parasite P falciparum, remain elusive We report here the ... than that of the A gambiae enzyme ( lmolÆ min)1Æmg)1) The possible physiological ⁄ functional significance and the implications of these differences between the Aedes aegypti and the A gambiae ... elusive We report here the identification, the bacterial heterologous expression and the biochemical characterization of 3-HKT from A gambiae These results represent the first biochemical report concerning...

Báo cáo khoa học: Biochemical analysis of the human EVL domains in homologous recombination doc

... contained the EVH2 domain (Fig 1A) EVL( 1–221) and EVL( 222– A B C We ﬁrst tested the ssDNA-binding and dsDNA-binding activities of EVL( 1–221) and EVL( 222–418), because the DNA-binding activity of EVL ... The EVH2 domain is responsible for the DNA-binding activity of EVL Preparation of EVL( 1–221) and EVL( 222–418) To identify the functional domain responsible for the recombination activity of human ... hand, the EVH1 domain binds to proteins containing the consensus sequence D ⁄ E FPPPPXD ⁄ E [21–23] The Pro-rich domain also binds to proﬁlin and proteins containing the SH3 and WW domains [17,24–26]...

Báo cáo khoa học: Analysis of the RNA degradosome complex in Vibrio angustum S14 potx

... degradation, the degradosome is also responsible for the processing of 5S ribosomal RNA and tRNAs, as well as the degradation of tmRNAs [4–8] The principal proteins comprising the degradosome include the ... degradosome complex in Vibrio angustum S14 have expanded on these results and shown that RhlB can interact with the CTH of V angustum S14 RNase E and the predicted RhlB-binding microdomain Interestingly, ... in V angustum S14 can self-associate Based on our observations, we present a model for the RNA degradosome complex in V angustum S14 which shows the positions of the microdomains in the CTH of...

Báo cáo khoa học: Biochemical characterization of the native Kv2.1 potassium channel ppt

... guide for biochemical purification of other potassium channels Results Expression and biochemical characterization of native Kv2.1 Regional distribution in various rat tissues of Kv2.1 channel protein ... from recombinant cell lines Here we report the biochemical characterization of Kv2.1 protein complexes The biochemical profile of the Kv2.1 potassium channel forms a foundation for subsequent large-scale ... shown) The behavior of Kv2.1 under different detergent treatments was 3745 Purification of Kv2.1 potassium channels Heterogeneity of Kv2.1 complexes In order to biochemically characterize the Kv2.1...

Báo cáo Y học: Brassica napus soluble epoxide hydrolase (BNSEH1) Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris docx

... step and to elute the recombinant enzyme in the elution step were determined in preliminary experiments Fractions were analysed for epoxide hydrolase activity and protein content based on the ... probably corresponding to the histidine and tyrosine residues, respectively [6–8] Several compounds were tested for potential inhibitory effects on the hydrolysis of TSO by the recombinant enzyme ... on recombinant BNSEH1 enzyme activity Enzyme activity was determined using 100 lM TSO in the presence of various inhibitors in 0.1 M potassium phosphate, pH 7, using 0.1 lg BNSEH1 enzyme The inhibitors...

Báo cáo khoa học: "Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ" potx

... This is the first study to examine the binding of DBA, SBA, Isolectin B4, WGA, PNA, and UEA-I in the vomeronasal organ of the horse We found unique characteristics of lectin binding depending cell ... binding; ++, >66% binding Lectin Isolectin B4 WGA ＋＋＋－＋＋－－－－－＋＋ PNA ＋－－－－ UEA-Ⅰ ＋＋－－－＋ Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ ... immunohistochemistry[7,12], the observed lectin-binding patterns of the equine receptor cells may reflect the various subtypes of the vomeronasal receptor cells in the horse The marmoset supporting cells showed positive...

báo cáo khoa học: "Characterization of the stress associated microRNAs in Glycine max by deep sequencing" pot

... 15.69% in the alkalinity induced library The relatively lower percentage of 24 nt reads indicates that more kinds of miRNAs are involved in the response of G max to alkalinity compared with other stress ... complete profile of miRNAs in Glycine max Keyword: deep sequencing, Glycine max, microRNAs, stresses associated, miRNA Background Terrestrial plants face serious abiotic stresses (e.g drought, salinity, ... Characterization of the stress associated microRNAs in Glycine max by deep sequencing Haiyan Li1, 2, §, Yuanyuan Dong1, §, Hailong Yin2, Nan Wang1, Jing Yang1, Xiuming Liu1, 2, Yanfang Wang1, 2, Jinyu Wu1,...

Báo cáo y học: "Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" pptx

... be involved in the induction of apoptosis, including the 7a and 7b proteins [18,46,47] To analyze whether the deletion in ORF 7b had any influence on its Amino acid variability in ORF 7b and ... overexpression of ORF 7a, ORF interferon induction the Influence on apoptosis and type I 7b, and ORF 7b with by Influence on apoptosis and type I interferon induction by overexpression of ORF 7a, ORF 7b, and ... sequences in GenBank, and in none of the SARS-like bat CoV However, deletions of the whole ORF 7b and beyond have been acquired by SARS-CoV during the SARS epidemic in humans [25-27] The ORF 7b protein...

Báo cáo khoa học: " Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" docx

... apoptosis, including the 7a and 7b proteins [18,46,47] To analyze whether the deletion in ORF 7b had any influence on its Amino acid variability in ORF 7b and RT-PCR analysis of ORF 7b in clinical ... sequences in GenBank (except in an independent sequence of the Frankfurt strain) , and in none of SARSlike bat-CoV sequenced in the ORF 7b region (Figure 1) To determine whether the deletion originated ... from the Frankfurt-1 strain (AB257344) shows a 45 nucleotide in- frame deletion in the predicted transmembrane domain (TMD) The drawing below the alignment panel represents the ORF 7b in recombinant...

Báo cáo khoa học: " The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek''''s disease viruses" doc

... presentation of the bi-directional different The the The schematic presentation of the bi-directional promoter and the parts and directions of the promoter in different constructs , indicates the deleted ... described[17] Construction of recombinant plasmids expressing CAT gene under the control of different promoters Construction of the bi-directional promoter was made by the use of a series of primers synthesized ... SM: The role of pp38 in regulation of Marek's disease virus bi-directional promoter between pp38 and 1.8-kb mRNA Virus Genes 2006, 32:193-201 Ding JB, Cui ZZ, Jiang SJ: The enhancement effect of...

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