characterization of the reservoir rock
Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx
Ngày tải lên :
14/02/2014, 03:20
... understand the relationships among the polymorphs. Heats
of solution of the various polymorphs need to be measured, in
order to prove the relative order of stabilities for these solid
forms. At the ... a wavelength of 1.540600 Å. The X-ray data was collected
using an MBraun curved PSD with a chamber depth of 1 cm.
The focal radius of the system was 57 mm, and the 5 cm length
of the PSD subtended ... samples
which were isolated. These results are summarized in
Table 4. In many cases the mixture of polymorphs can be
accounted for by the addition of the DSC results of the
component pure polymorphs....
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
Ngày tải lên :
16/02/2014, 09:20
... whether these cytokines are
produced by CD4
+
CD25
+
Foxp3
+
T
reg
or whether
they induce the production of these cytokines from
another population of cells [20].
To date, our knowledge about the ... are
important in the generation of the correct class of
antibodies by B-cells, and for the elimination of
extracellular pathogens, such as helminths and other
extracellular parasites [2]. Th17 is the most ... zffoxp3-R2 for
foxp3. These primers amplified part of the initial predicted
sequence which contained the majority of the open reading
frames of zebrafish stat6, t-bet and foxp3 to check they were
correct....
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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
Ngày tải lên :
18/02/2014, 18:20
... between the two
steps of the TES reaction
The substrate guanosine to be excised (G
1
) and its
2¢-OH group are required for the second step of
TES [48], similar to (if not the same as) the role of
the ... that there is room for improve-
ment in terms of the rate of reaction. This would be
beneficial not so much in terms of the rate of the over-
all reaction, as the cleavage reaction is not the limiting
step ... of
the xG in the second step of self-splicing [48–56].
This suggests that the guanosine to be excised is
likely binding to the GBS of the ribozyme for the
exon-ligation step of TES. In the substrate-cleavage
step,...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
Ngày tải lên :
18/02/2014, 18:20
... analyses of the predicted
amino acid sequence showed that the C-terminal half
of the RepA of the pIT3 plasmid is sequence-similar to
the helicases of the phage-encoded superfamily III pro-
teins. The ... probably comparable
with the two-metal-ion mechanism of both RNA and
DNA synthesis [17].
In a previous study, we reported the findings of an
analysis of the complete sequence of the cryptic plas-
mid ... subunit of archaeal primases and the active
site of the X family of polymerases [23]. Indeed, unlike
the pRN1 prim–pol protein whose motif is DXE D,
the Rep245 protein, the X family of DNA polymerases
and...
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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
Ngày tải lên :
19/02/2014, 07:20
... of the first 88
amino acids of PPI1 may participate in the interaction
with the H
+
-ATPase and makes PPI1
588
His
6
a suitable
tool to study the mechanism of action of PPI1.
The analysis of the ... 14-3-3s.
Results
The C-terminus of isoform 1 of the PM H
+
-ATPase
of A. thaliana (AHA1) interacts with the first 88 amino
acids of PPI1 [22], indicating that the PM H
+
-ATPase
binding site of PPI1 is ... Thus,
all the following experiments were performed with
PPI1
588
His
6
.
The analysis of the effect of PPI1
588
His
6
on the
dependence of PM H
+
-ATPase activity on the concen-
tration of MgATP...
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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx
Ngày tải lên :
19/02/2014, 12:20
... min, the absorbance of
the supernatant was read at 363 nm. The absorbance of
the reduced form of APAD (APADH) was linear over the
2–100 l
M
range of glutamate with a molar extinction
coefficient of ... complement the defect. The
relationship of all of these genes remains to be clarified.
Expression and purification of Sno1p and Snz1p
In light of the similarity in the amino acid sequence of
Sno1p ... together in the
stationary phase of yeast cells [8] and the result of two-
hybrid analysis [23], they may form a complex. To assess
these possibilities, we embarked on a characterization of
the...
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Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
Ngày tải lên :
19/02/2014, 12:20
... required
for the protonation of the amine groups of samples. The
number of equivalents of acid groups was calculated using
the formula: [NH
3
+
](mequiÆkg
)1
) ¼ 1000 · molarity
(moles/L) · volume of titrant ... (mL)/mass of sample (g)
and the level of deacetylation was calculated by comparison
between the number of free amino groups (per unit weight
of the sample) and the equivalent weight of GlcN [23].
Circular ... 200 mg KBr
and 40 mg of the mixture was pelletized. The reproducibility
of the spectra was verified on two preparations and the
degree of acetylation (DA) was determined using the formula
(A
1655
Æcm
)1
/A
3450
Æcm
)1
)...
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Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc
Ngày tải lên :
19/02/2014, 17:20
... for
which the novel pattern is located in the third loop.
The reason for the similarity and location of these pat-
terns could be a result of short evolutionary time
rather than gain of new features ... suggest-
ing that the origin of these forms dates back to before
the occurrence of vertebrates, i.e. more than 500 mya.
This dates the differentiation of the MAPEG forms
back to the late Cambrian ... hydrophobic seg-
ment. All of the remaining patterns are located at the
end of first loop (Fig. 6). Both the first and third loop
are located on the cytosolic side of the membrane and
are regions...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
Ngày tải lên :
21/02/2014, 00:20
... PHO (abc) protomer. The hypothesis of a
direct PHI–phenol interaction is quite unlikely, because of
the fact that the addition of phenol does not alter the
emission spectrum of PHI. Moreover, PHI ... equilibrium. As shown in the inset,
the progressive decrease of the molecular ellipticity at
k ¼ 200 nm (the absorption region of the peptide bond)
reflects the occurrence of a transition between ... 1. The 18 kDa protein
present in the elution pattern was identified as the PHI
component on the basis of its ability to complement the
Fig. 1. SDS/PAGE of PHI at different steps of purification. The
numbers...
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Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
Ngày tải lên :
21/02/2014, 03:20
... CCAAT box at 324 bp upstream of
the major transcription start site. The presence of a CCAAT
box and the absence of a TATA box seem to be one of the
characteristics of the mouse integrin a3 gene.
We ... We
previously reported the structures of the mouse a3integrin
subunit gene including the exon/intron organization and the
alternative exon usage for the generation of variants of the
a3 subunits (a3A ... upstream of the SacI) sites (Fig. 1).
Sequence analysis of the 5¢-flanking region and
determination of transcription start sites of mouse
integrin a3 subunit gene
The nucleotide sequence of the 0.5...
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Tài liệu Báo cáo Y học: EPR characterization of the mononuclear Cu-containing Aspergillus japonicus quercetin 2,3-dioxygenase reveals dramatic changes upon anaerobic binding of substrates potx
Ngày tải lên :
22/02/2014, 07:20
... different
flavonoids. The addition of a flavone (apigenin), of a
flavanonol (taxifolin) and of a flavan-3-ol (epicatechin)
(Fig. 8) did not alter the EPR spectra of the enzyme
indicating the absence of binding to the ... definition of some of the catalytic steps. Binding of
the various flavonols to the active site metal centre is found
not to require the presence of dioxygen and occurs without
formal reduction of the ... perturbation of the axial symmetry
of the tensor, whereas axial symmetry, i.e. g
xx
% g
yy
<< g
zz
is typically found for type 2 sites. The grouping of the
g-values and the line-shape of the spectrum...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... For
comparison, the amount of GroEL was determined in the
same extracts using anti-GroEL Ig. It has been previously
shown that at the beginning of the stationary phase the rate
of synthesis of heat-shock ... respectively. In order to
estimate the ratio between the amounts of UP12 and the
total amount of protein in the extracts, a series of samples
containing determined amounts of the purified UP12 were
separated ... the deletion of the UP12 encoding gene increased
the sensitivity of the mutant to CCCP (Fig. 6), it did not have
any detectable effects on the sensitivity to mitomycin C,
unlike in the case of...
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Đề tài " Numerical characterization of the K¨ahler cone of a compact K¨ahler manifold " doc
Ngày tải lên :
05/03/2014, 23:20
... components of the open
set
P = {det(α) > 0} of hermitian forms of positive determinant (the other
components, of course, are the sets of forms of signature (p, q), p + q = n, with
q even).
One of the ... wish to investigate the behaviour of the K¨ahler cones
K
t
of the various
fibres X
t
= π
−1
(t), as t runs over S. Because of the assumption of local
triviality of π, the topology of X
t
is locally ... on the subbundle t → H
p,q
(X
t
, C).
The main result of this section is the proof of Theorem 0.8, asserting that
there is a countable union of analytic subsets S
=
S
ν
S outside which the
K¨ahler...
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Báo cáo khoa học: Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp. BH72 ppt
Ngày tải lên :
07/03/2014, 02:20
... inactivation
of the enzyme. Thus, the results obtained in the pres-
ent study extend our knowledge of the nitrogenase
post-translational modification system outside of the
a-class to other members of the ... under these
conditions. The strong effect of TFA on the arginine-
specific ADP-ribosylation might be caused by the
high degree of acidity of this acid with its pK
a
value
of 0.26 compared to the other ... one of
the most abundant enzymes in the biosphere [1,2]. It
consists of the Fe-protein (dinitrogenase reductase,
also referred to as NifH), an a
2
dimer of the nifH gene
product and of the MoFe-protein...
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Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt
Ngày tải lên :
07/03/2014, 03:20
... solution of the HPLC-purified compound, and then
incubating for 20 min at 50 °C. The product was then par-
titioned against petroleum ether ⁄ diethyl ether 1 : 4 (v ⁄ v).
The identification of the oxime ... preference of OsCCD1 for the
C5¢–C6¢ and C7¢–C8¢ double bonds depends on the
nature of the substrate.
The cleavage of a sole double bond in the cyclic moi-
ety of 3-OH-c-carotene and of monocyclic b-apocarote-
nals ... The
occurrence of the C
17
dialdehyde confirmed the novel
site at the C7¢–C8¢ double bond observed with apoly-
copenals and implied the formation of geranial.
Accordingly, the GC-MS analyses of...
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- truyện kinh dị allies of the night
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- truyện hunters of the dusk
- the holder of a recreational rock
- rocks landscapes resources of the wet tropics
- the love of your life rock song 2013
- the love of your life rock song
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