Báo cáo y học: "RNA interference pinpoints regulators of cell size and the cell cycle" doc

... Drosophila cell cultures treated withdouble-stranded RNA were analyzed by flow cytometry, providing a wealth of new information and identifying 488 regulators of the cell cycle, cell size, and cell ... detectable pheno-types. Surprisingly though, they primarily affected the cell cycle rather than cell size. Activation of this pathway in vivo,through the mutation of negative regulators or the over-expression ... over-expression of positive regulators, decreases the proportion of cells in G1 and increases the proportion of cells in S phase and G2/M [11-13]. Conversely, inhibiting the pathwayincreases the proportion...

Báo cáo y học: "RNA interference: more than a research tool in the vertebrates'''' adaptive immunity" ppsx

... the role of RNA in many facets of biology,illustrating the complexity and the dynamic interplay of cellular regulation. As plants and invertebrates lack the protein-based adaptive immunity that ... pathway. The biogenesis of RNA silencing transcripts can be derived from either the host cell nucleus mRNA pathway to yield miRNA or the cytoplasmic double strand RNA to yield siRNA. HIV-1 and ... in the nucleus, which are then cleaved by a RNaseIII-like enzymeknown as Drosha to generate the pre-miRNA. The pre-miRNA is then transported into the cytoplasm aided byExportin 5. Once the...

Báo cáo y học: "RNA interference of argininosuccinate synthetase restores sensitivity to recombinant arginine deiminase (rADI) in resistant cancer cells" pps

... 0.05). Cell viability and cell cycleTo observe the effect of the combination of ASsiRNA and rADI i n HeLa and MCF-7 cells, cell viability and cell cycle were analyzed by MTT and flow cytometry,respectively. ... cytometry,respectively. The results of the cell viability (Figure 3)show that only the combination of ASsiRNA and rADI(Figure 3a) significantly inhibited proliferation and survi-val in HeLa cells. Cell viability ... MCF-7 cells. However, autophagy is not nor-mally occurred in a wide variety of cells. Accordingly, the different cell lines may also explain the discrepancy.ConclusionsDe novo arginine synthesis...

Báo cáo y học: "RNA interference against polo-like kinase-1 in advanced non-small cell lung cancers" doc

... by a Grant-in-Aids for Scientific Research from the Ministry of the Education, Culture, Sports, Science, and Technology of Japan.Author details1Department of Transfusion Medicine and Cell Therapy, ... Cell Therapy, Kyoto UniversityHospital, Kyoto, Japan.2Division of Internal Medicine, Kyoto Second RedCross Hospital, Kyoto, Japan.3Department of Molecular Cell Ph ysiology,Kyoto Prefectural ... establish the pharmacokinetics and pharmacodynamics of siRNAson the administration are necessary steps in the potentialapproval of siRNA as a tool for cancer thera py. To maxi-mize efficacy and...

Báo cáo y học: "RNA interference for CFTR attenuates lung fluid absorption at birth in rats" doc

... rely oncultured cells of uncertain phenotype and did not address the possibility that fluid transport may involve multipledifferent epithelial cell types, including alveolar epithelialtype ... deficiency of this channel mayalso affect the function/expression other channels. Poten-tially this may be explained as that ion flow through the channel, either partially or wholly, depends on the ... in the study. The rats were housed separately in their cages in atemperature- and humidity-controlled environment (20 ±2°C and 55 ± 10% relative humidity). The rats were keptat a 12:12 h day-night...

Báo cáo y học: "RNA interference: a multifaceted innate antiviral defense Ajit Kumar" ppsx

... (pri-miRNA) and then proc-essed by the nuclear enzyme Drosha into shorter stem-loop structures (pre-miRNAs) that are exported from the nucleus to the cytoplasm, where they are cleaved by Dicerto yield ... expression of large T antigen late in the viral replica-tive cycle [14]. Since SV40 large T antigen is the major tar-get of the host cytotoxic T lymphocyte (CTL) response, itsdownregulation by the ... miRNAs[6].Unlike the perfect base-complementarity of the siRNA'guide' strand and its target mRNA, miRNAs bind their tar-get mRNAs primarily through their 5' nucleotides 2–7,also known as the...

Báo cáo y học: "RNA interference has a role in regulating Drosophila telomeres" potx

... million years since the separation of the genus Drosophila [17]. Their regulation by the RNAi machinery could simply be a reflection of theirretrotransposon nature. On the other hand, there is ... increase both the abundance of HeT-A and TART RNA and their frequency of transpositionto broken ends [9]. In contrast, loss of one copy of either of the DNA repair genes Ku70 or Ku80 strongly increased ... havemany functions, yet we understand little about how theyactually accomplish any of them. The RNA interference machinery affectstelomere elongation in DrosophilaAlthough the mechanism of transposition...

Báo cáo y học: "RNA interference is not involved in natural antisense mediated regulation of gene expression in mammals" docx

... splice forms of HIF [14-16]. Specifically, it has beenhypothesized that the antisense molecule may destabilize onesplice variant of HIF mRNA and shift the balance in favor of the other variant ... artificialRNA duplexes and then transfected them into the cells. Toinvestigate the presence of RNA duplexes in transfected and untreated cells, cytoplasmic lysate was isolated and subse-quently treated ... Biology 2006, Volume 7, Issue 5, Article R38 Faghihi and Wahlestedt http://genomebiology.com/2006/7/5/R38Genome Biology 2006, 7:R38tion of RNAse A and T to assess the specificity of the probeand...

Báo cáo y học: " Post-traumatic glioma: Report of one case and review of the literature"

... with a secondary reparative process; (3) the location of the impact and the occur-rence of the tumor corresponded exactly one to the other; and (4) there was a more than one year interval between ... or supple-mented by imaging criteria. CT/MRI, which elegantly reveal the severity and location of the traumatic brain injury and the subsequent formation of a neoplasm at the same site, can ... histologically verified; it must be emphasized, how-ever, that CT scan documented the absence of tumor at the time of the brain injury. We suggest that these conditions for the accep-tance of a causal...

Báo cáo y học: "Advances in Molecular Diagnosis of HBV Infection and Drug Resistance"

... decrease the number of handling steps, reduce contamination, and increase throughput and the accuracy of quantification will further enhance the utility of these assays [2,5]. Given the varied technologies ... M, Miyano Y, et al. Emergence and takeover of YMDD motif mutant hepatitis B virus during long-term lamivudine therapy and re-takeover by wild type after cessation of therapy. Hepatology 1998;27:1711-1716. ... they may be well suited for the study of multiple mutations on the same genome [41]. However, as the number of clinically relevant mutants is presently relatively limited, the development of...

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