... amylin to evaluate its effect onamylin aggregation. Samples were incubated at 37 °C for 72 h with shaking, and were taken for ThT assays, light scat-tering assays and HPLC analysis at selected ... insulin may be not only a natural inhibi-tor of amylin aggregation, but also a contributor tothe amyloid formation and pathogenesis of T2D. Wealso found that the promotional effects were caused ... componentof islet amyloid [15]. Thus, extracellular amyloid,which is the major part of islet amyloid, may possiblybe formed by more complicated mechanisms. However,insulin may still act as a contributor...