... cells in culture and stainednegatively for CD20 and CD3 and positively forCD14 and VLA-4 by flow cytometry (data notshown). In addition, viability testing indicatedthat by day 6, all B cells ... 217025,26clearly had an effect,decreasing cell proliferation (as measured by [3H]-thymidine incorporation) by 35% and the produc-tion of IgM and IgG by the isolated tonsillar cells by 45 to 75%. ... associated B lymphocytes.FDCs were plated in six well plates in completemedium; after 3 days, the nonadherent B cellswere removed, and the remaining FDCs were kept in culture. They were fed by the addition...