Báo cáo y học: "Identification of candidate predictive and surrogate molecular markers for dasatinib in prostate cancer: rationale for patient selection and efficacy monitoring" doc

... targets, including Abl, c-kit and PDGFR kinases, showed minimal efficacy in early clinicaltesting with a small number of patients [19,20]. Identification of these preclinical candidate markers and ... R255ResearchIdentification of candidate predictive and surrogate molecular markers for dasatinib in prostate cancer: rationale for patient selection and efficacy monitoringXi-De Wang, Karen Reeves, ... Additionally, EphA2, a target of dasatinib, is a candidate predictor of efficacy in both prostate and breastcancer. Finally, the observed sensitivity to dasatinib of pros-tate cancer cell lines with...

... liverFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●YYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYY Y YYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYY Y YY Y YYYYYYYYYYYYYYYY Y YYYYYYYYYWetterbom et al. Genome Biology 2010, 11:R78http://genomebiology.com/2010/11/7/R78Page ... predicted in the brainF sample and 4,279 in liverF,with as many as 90% of them mapping exactly to an exon-exon boundary in a known gene. The remaining 10% arethe most interesting, since they include ... TRs in brain and 61,920 in liver. TRs were often found in clusters, thusindicating that closely spaced TRs originated from thesame transcript and with increasing sequencing depthmany of the...

... Culture and Stem cell culturePrimary mixed glial cultures were prepared from thebrains of newborn rats > 3 days old by dissociation of whole cortex in trypsin, then cultured in poly-D-lysinecoated ... specificity,cotranslational packaging and competition for limitingGag polyprotein [13]. These differences in the location of the major packaging determinants may contribute to theability of viral mRNA to be ... of experimentally inducedParkinson's disease [6]. Packaging of unspliced vectormRNA in the producer cell line is a key part in process of lentiviral vector production and measures to increase...

... prescription of ChEIs has not yet been studied systematically. Therefore the main question of this study is: Do geriatric comorbidity and polypharmacy influence the prescription of ChEIs in patients ... galantamine) in the incidence year. Our main independent variables were polypharmacy and measures of geriatric comorbidity. The concurrent use of multiple drugs is often termed polypharmacy, but ... 2011-07-09. (Archived by WebCite® at http://www.webcitation.org/602utmyNL). 3 German Society for Psychiatry and Psychotherapy and German Society for Neurology: S3-Guideline Dementias (in German); 2009....

... (IC50) of full-length DNA synthesis.Determination of steady-state kinetic parametersThe Km for dATP and the Ki for TFV-DP were determinedby filter binding assays as described previously [36]. In brief, ... resolved in 6% polyacrylamide-7M urea gel and ana-lyzed by using the Molecular Dynamics Typhoon Phos-phorimager system (GE Healthcare). To study the effect of mutated RTs on the initiation of synthesis ... significantlyimpaired in specific activity compared with wild-typeenzyme, with K65R exhibiting only 46%–50% of wild-type activity and K65R+M184V RT exhibiting only ≈ 30% of wild-type activity. The...

... a candidate citrullinated protein by immunoblotting lysates of monocytic and granulocytic HL-60 cells treated with peptidylargininedeiminase. In an initial screen of serum samples from fourpatients ... that stainedpositively for enolase, although the intensity of the stainingindicated that levels of citrullination were relatively low. Immu-noblotting of synovial cell lysates from three patients ... deiminated and undeiminated α-eno-lase in RA patients and healthy controlsPrevalence of antibodies against deiminated and undeiminated α-eno-lase in RA patients and healthy controls. Immunoblotting of...

... Robert Haynes for the countless hours spent in caring for and maintaining precise records of these RI lines and Sung Kim for his help in analyzing the function and pathway associations of selected ... Paraffin-embedded sectionswere de-paraffinized by immersing in xylene, followed by dehy-drating in ethanol. The tissue sections were prepared and stained with hematoxylin and eosin dye (Histology ... balance of IL-6 and transforming growth factor-beta influenced by retinoicacid. Thus, the intricacy and balance between OX40L, theretinoids, proinflammatory cytokines, and development of Tregcells...

... of up-per/lower endoscopy in outpatients with iron defi-ciency anemia. The aim of our study was to investi-gate the incidence of GI pathological findings in symptomatic and asymptomatic patients ... deficiency. All patients were interrogated and examined according to Form-1 in Figure 1. This form developed by us for this study. The presence of dyspeptic com-plaint and its severity calculated ... evaluate the diagnos-tic yield of endoscopy in patients with IDA and to define predictive factors of gastrointestinal (GI) le-sions causing IDA and identify clinical and biochem -ical variables...

... use of yeast two hybrid system, which is a genetic assay for in vivo detection of interactions of proteins, enables identification of protein-protein interactions between a soluble bait and ... and its interacting prey [5]. However, such a screening system is not suitable for the analysis of interaction between membrane bound proteins. The split-ubiquitin yeast two hybrid system has ... g for 10 minutes and the supernatant discarded. The pellet was resuspended in 5ml 0.5X YPDA/Kanamycin (50µg/ml) and combined into a total volume of 10ml. To calculate the mating efficiency,...

... 2Flow cytometric analysis of combinations of progenitor markers on freshly isolated and culture expanded chondrocytes from osteoarthritic cartilageFlow cytometric analysis of combinations of progenitor ... penicillin, 100 µg/ml streptomycin; Bio-chrom, Berlin, Germany); fine mincing and digestion with0.2% pronase (Roche, Mannheim, Germany) for 45 min at37°C; and two further washes followed by enzymatic ... between isotype and antibody staining. Amaximum of 2% positive cells by staining with isotype anti-body mouse IG1 or IG2 conjugated with FITC, PE or biotinwas allowed and therefore was used...