... ells were l ysed by three cycles of freezing at -80°C and thawing. Unpackaged DNA wasremoved b y adding 50 μl of Benzonase (Nov agen) andincubating at 37°C for 2 hours.Determination of viral ... Preferential integration of adeno-associated virustype 2 into a polypyrimidine/polypurine-rich region within AAVS1. J Virol2007, 81:9718-9726.45. Huser D, Weger S, Heilbronn R: Packaging of human ... Weitzman MD, Kyostio SR, Carter BJ: Identification of a DNA-binding domain in the amino terminus of adeno-associated virus Repproteins. J Virol 1993, 67:997-1005.23. Wonderling RS, Kyostio SR,...