... (1989) Gly-cosyltransferase assays using p yridylaminated acceptor: N-acetyl-glucosaminyltransferase III, IV, and V. M ethod s Enzymol. 179,397±408.21. Nishikawa, A., Fujii, S., Sugiyama, T. ... ¯uorescentreagent, 2-aminopyridine and sodium cyanoborohydride[24]. After pyridylamination, the excess reagents wereremoved b y gel ®ltration with H W-40F (Toy opearl, Tosoh).The pyridylaminated sugar ... H., Yonezawa, T., Tanigu-chi, N. & Tarui, S. (1990) Carbohydrate analysis of immuno-globulin G myeloma proteins by lectin and high performanceliquid chromatography: role of glycosyltransferase....