... dimethylsulfide; EDT,ethanedithiol; hCt, human calcitonin; MBHA, 4-methyl benzhydryl-amine resin; Mtt, 4-methyltrityl; Pip, 2-phenylisopropyl;OFm, fluoren-9-yl methylester; sCt, salmon calcitonin, ... theirchirality are strongly associated with receptor bindingaffinity (c) inversement of the chirality of residues 18 and19 strongly reduces the binding affinity of hCt and (d) theAsp17,Lys21-lactam ... massspectrometry (MALDI-MS) with a Kratos CompactMALDI I (Shimadzu Europe, D uisburg, Germany) anda-cyano-4-hydroxycinnamic acid as matrix. Purity of theHPLC purified peptides were also confirmed by analyticalHPLC...