Báo cáo y học: "If good things come from above, do bad things come from below" ppt
Báo cáo y học: "If good things come from above, do bad things come from below" ppt
... cysts. These data provoke questions about the nature and role of bone marrow lesions.© 2010 BioMed Central LtdIf good things come from above, do bad things come from below?David M Findlay*See ... lubricin, come from above. Is it possible that factors which lead to the destruction of cartilage come from below in the subchondral bone? The recent acquisition of tools to probe early events ... identify the disease and to follow it longitudinally. Since the main early symptom is pain, clinical trials of new therapies have been problematic. Animal experiments have been bedevilled by a...
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Báo cáo y học: "If you don''''t believe it, it won''''t help you": use of bush medicine in treating cancer among Aboriginal people in Western Australia" pps
... methodology to acknowledge cultural difference and build understanding. BMC Medical Research Methodology 2009, 9:60.36. Braun V, Clarke V: Using thematic analysis in psychology. Qual Res Psychology ... worried taking any ofthat "Secrecy and mystery abound in the Aboriginal commu-nity about the use and availability of bush medicine. Thisinevitably means poor availability of accurate informationregarding ... It may not save your life, but it will help you, even if it's only in a men-tal or an emotional way of help. So, I really do believe that it would help, and have just having somebody there...
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Báo cáo y học: "A folate-rich diet is as effective as folic acid from supplements in decreasing plasma homocysteine concentrations"
... former study of CAD patients from our institution [17], which may be explained by the fact that patients from this study were selected on the basis of high tHcy levels. A statistically-significant ... are required daily to treat hyperhomocysteinemia. To reach this amount by dietary changes alone may be difficult because food has a low folic acid content and bioavailability. No studies have ... daily intake of a variety of folate-rich foods is as effective as folic acid from supplements for lowering plasma tHcy concentrations. A high percentage of patients with CAD has hyperhomocysteinemia...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... correlate exactly with the mobilities ofdi-, mono- and unglycosylated forms of recombinantcystatin F previously characterized by enzymatic removalof N-linked carbohydrate and carbohydrate compositionanalysis ... compositionanalysis [5]. It is also noteworthy that all three forms werepartially purified by their affinity to Cm-papain, stronglyindicating that the carbohydrate side chains do not affect theenzyme-binding ... the cystatin F produced by U937 cells isretained intracellularly or is taken up by the cells immedi-ately following secretion. Accordingly, the relatively lowamount of cystatin F produced by...
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Tài liệu Báo cáo Y học: The Ikaros family protein Eos associates with C-terminal-binding protein corepressors pptx
... vector pGL2-TK (Promega).Yeast two-hybrid and pull-down assaysThe Clontech yeast two-hybrid system was used accordingto the manufacturer’s instructions. The prey and baitplasmids used are ... 2002The Ikaros family protein Eos associates with C-terminal-bindingprotein corepressorsJose´Perdomo and Merlin CrossleyDepartment of Biochemistry, G08, University of Sydney, NSW, AustraliaEos ... remodeling complexes in lymphocytes.Immunity 10, 345–355.21. Turner, J. & Crossley, M. (2001) The CtBP family: enigmatic andenzymatic transcriptional co-repressors. Bioessays 23, 683–690.Ó FEBS...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... enzyme, aplausible explanation may be that 4-phosphorylated UTPby itself acts as a weak activator of glutamine hydrolysis.This activation is greatly enhanced by GTP binding to theenzyme. From ... glutamine hydrolysishas been shown to be greatly stimulated by bicarbonate-dependent ATP hydrolysis, indicating that for this enzymethe phosphorylated amino acceptor intermediate, carbonylphosphate, ... the same type of allosteric activation ofglutamine hydrolysis takes place by the phosphorylation ofUTP on CTP synthase, only that for CTP synthase thisallosteric effect exerted by the amino...
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Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx
... recombinant aA-crystallin and its two domains. Bi ochim.Biophys. Acta 1130, 267–276.8. Augusteyn, R.C. (1998) a-Crystallin polymers and polymerization:the view from down under. Int. J. Biol. Macromol. ... variants produced byp26-ND60 and p26-alpha were pr esent but recognizedpoorly by antip26 antibody. To determine if recognition ofp26-ND60 and p26-alpha encoded polypeptides by antibodyto p26 was ... SDSpolyacrylamide gel treatment buffer. Twenty microliters ofeach sample was then electrophoresed in 12.5% SDSpolyacrylamide gels, blotted to nitrocellulose and probedwith antibody to p26....
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Báo cáo Y học: Two different E2F6 proteins generated by alternative splicing and internal translation initiation ppt
... confirmed by FACS analysis.RNA isolationTotal RNA was isolated from primary MEFs with theRNeasy kit (Qiagen) according to the manufacturer’sinstructions. To isolate RNA from primary mouse tissues,an ... c-myc, which is mediated by an internal ribosome entrysite during apoptosis [18], and efficient translation ofvascular endothelial growth factor from an internalribosome entry site during hypoxia ... ona Dynex Luminometer. Luciferase activity was normalizedto b-Gal activity to correct for differences in transfectionefficiency.Primer extensionTotal RNA was extracted from mouse embryonic...
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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
... permethylated total N -glycans enzymatically liberated from huma n HCI I isolated (A) from human serum or ( B) produced by genetically engineered CHO cells. The following complex type carbohydrate ... permethylation of oligosaccharidesThe enzymatically liberated N-glycans were reduced andpermethylated as described previously [27].MALDI-TOF MSThe reduced and carboxamidomethylated tryptic ... of fully glycosylated (78 kDa) and completely u nglycosylated(64 kDa) HCII, respectiv ely.Ó FEBS 2002 Heparin cofactor II: N-glycans and sulfation (Eur. J. Biochem. 269) 987HCII m ay provide...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
... alyase-isomeraseforthephotoactive31-Cys-a84-phycoviolobilinchromophore of phycoerythrocyanin. Biochemistry 40, 12444–12456.Ó FEBS 2002 Analyses of a-phycoerythrocyanin peptides (Eur. J. Biochem. 269) 5055Optical spectroscopyThe ... phycobiliprotein lyase: both the attachment of phy-cocyanobilin and the isomerization to phycoviolobilin are cata-lyzed by the proteins PecE and PecF encoded by thephycoerythrocyanin operon. FEBS ... Germany;2Botanisches Institut der Ludwig-Maximilians-Universita¨t, Mu¨nchen,GermanyThe a-phycoerythrocyanin subunits of the different phy-coerythrocyanin complexes of the phycobilisomes from the...
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