báo cáo khoa học: " Bioinformatic analysis of the CLE signaling peptide family" ppt
báo cáo khoa học: " Bioinformatic analysis of the CLE signaling peptide family" ppt
... CLE sequences from various plant species CLE3 4, CLE3 6, CLE6 4, CLE7 8, CLE8 0, CLE1 17, CLE1 18 and CLE1 63,due to the redundancy in the conserved domain. Because the CLE domain that was used for clustering ... of the Multidomain CLE sequencesFigure 1Multidomain CLE sequences. The potential multidomain CLE signaling peptides CLE7 5, CLE7 6, CLE6 8, CLE3 1 and CLE3 0 are represented. The figure is a scaled ... Charac-terized CLE members HgCLE (CLE4 7), TDIF (CLE4 9) and ZmESR (CLE1 43 CLE1 47), as well as the known orthologs CLV3/FON4 and CLE1 9/BnCLE19 (CLE1 62) are highlighted with red stars. The single CLE member...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... related to the reduction of the PQ pool.ResultsAs reported in the literature, the I step of the O–J–I–Pfluorescence transient cannot be clearly distinguishedby visual analysis of the FI traces ... amplitudes and rate constants of the two other phases. The above should be gov-erned by the balance between the rate of reductionand oxidation of the acceptor side of PSII by the avail-able electron ... half-time of the J–I phase were bothdecreased with DCMU, demonstrating that the J–I riseis not directly related to the reduction of the PQ pool.A further analysis of the influence of PQ reductionon...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... connected to the DEC1 E-boxes(Fig. 2), while deletion of the Orange domain ( DEC1DOr-ange) had no effect on the suppressive activity, indicating the importance of t he bHLH region of DEC1. The HXXXXXXXR ... conclude that the basic region, p artic-ularly the conserved A rg65, but not His57, is essential for the suppressive activity of DEC1.Determination of the binding domain of DEC1 to BMAL1 The interaction ... network of the circadian clock system[11,12,14]. In fact, the circadian rhythms of Dec1 and Dec2expression have been shown to be completely disrupted in the SCN a nd some o ther tissues of Clock/Clock...
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Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf
... Addition of the L2 peptide (dansyl-GSKL) was safelypossible due to the conserved binding site and the presence of a YQSKL pentapeptide in the modellingtemplate. Modelling of the longer peptide ... investigate the binding of the dansylated PTS1 peptide L2 to HpPex5p and correlate the fluorescence data withstructural data, a molecular model of the HpPex5p wasbuilt based on the known structure of the ... residues in the C-terminus of HpPex5p to the dansyl cap in the PTS1peptides, the distance between the dansyl cap in L2 and the three tryptophan residues in the model were measured. The values...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... of the structure of NADP+-bound FeRrevealed that the nicotinamide moiety of NADP+faces the re-side of the isoalloxazine ring, and that the 2¢-P-AMP moiety is held in the groove between the ... direction along the b10 of the other mono-mer, forming an anti-parallel intermolecular b sheet(Fig. 2A). The subsequent residues of the loop (Ala7–Pro13) turn into the interior of the dimer interface,and ... linearregression analysis of the logarithms of the concentrationratios for oxidized and reduced forms of the mediatorversus that of recombinant flavoredoxin.Reduction titration of flavoredoxin by NAD(P)HThe...
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Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
... proteins. The 3D arrangement of the binding sitesfor TIPRL and a4 on the surface of PP2Ac shows thatthey are in close proximity, but not overlapping, whichallows the assembly of the TIPRL:PP2Ac:a4 ... indicates the elution positions of molecular mass markers are shownabove the panels. The profiles of the three proteins overlap over a region that coincides with the expected molecular mass of the ... at the level of complexstability, but rather at the level of activity or substratespecificity. The apparent discrepancies between studiesin yeast and mammalian cells indicate that the TORsignaling...
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Báo cáo khoa học: Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris ppt
... incomplete process-ing of the signal peptide was also reported [16,17]. The uncleaved part of the a-mating sequence at the N-ter-minus as well as the histidine tag at the C-terminus of the recombinant ... lines and the curved blue arrows indicate the overall orientation of the cavities. (C), (F), (I) Ribbon diagrams at the top of the Man-binding lectins show-ing the overall topography of the carbohydrate-binding ... 2). The fusion protein wassuccessfully expressed in the Pichia strain X-33.Because of the presence of the a-mating sequencefrom Saccharomyces cerevisiae at the N-terminus of the construct, the...
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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot
... introducingfeatures of the consensus motif into the Zm-p60.1scaffold, an analysis of structural and catalytic prop-erties of the mutants, and simulations of the sub-strate–enzyme interactions of the wild-type ... F200Kand P2. Furthermore, the F193A and W373K muta-tions do not result in any change in the thermostability of the enzyme (Table S2), and thermal unfolding of the wild-type and both the F193A and ... by the W373K mutation indicate a pre-viously unrecognized function of W373 in the determi-nation of the catalytic rate of the enzyme, albeit onethat is consistent with the involvement of F193–agly-cone–W373...
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Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot
... Amilestone in the study of peptide s ynthetases was the determination of the A-domain structure of GrsAPhe-A [8].Aspects of speci®city and binding were superimposed onother A-domains and with the help ... selectivity of the A-domains [9]simultaneously determines the primary sequence of the product. Subsequently the a ctivated amino acid i s tetheredto the terminal thiol moiety of a 4¢-phosphopantetheinyl(Ppant) ... dipeptideformation The formation of the dipeptide was analyzed using G rsAPhe-ATE and the different C-domain mutants of TycB1Pro-CAT. To ensure a complete acylation of the p eptidesynthetase...
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Báo cáo khoa học: Biochemical analysis of the human EVL domains in homologous recombination doc
... in the NdeIsite of the pET15b vector (Novagen, Darmstadt, Germany).In this construct, the His6-tag sequence was fused to the N-terminus of the protein. The EVL fragments were expressedin the ... described previously [28,29]. The con-centrations of the RAD51 and EVL proteins were determinedwith the Bradford method, using BSA as the standard. The concentrations of the EVL(1–221) and EVL(222–418) ... clarify the contributions of these proteins in the HRR pathway.During the cytoplasmic actin-remodeling process, the ENA ⁄ VASP family proteins bind to both the barbed end and sides of actin...
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