Báo cáo khoa học: "Identification and characterization of a new E3 ubiquitin ligase in white spot syndrome virus involved in virus latency" pot

... CentralPage 1 of 8(page number not for citation purposes)Virology JournalOpen AccessResearchIdentification and characterization of a new E3 ubiquitin ligase in white spot syndrome virus involved ... threeWSSV403 contains a RING domainFigure 1WSSV403 contains a RING domain. (A) Schematic representation of WSSV403 protein by SMART program. RING domain is from 329 aa to 370 aa. Green bars indicated coiled ... ubiq-uitin-dependent processes to modulate cellular signalingpathways involved in latency regulation [25]. As a RING-containing E3 ubiquitin ligase, WSSV403 is able to inter-act with its substrates...

Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-afﬁnity substrate for H+/peptide cotransporters pdf

... sub-strates of H+⁄ peptide cotransporters, such as Gly-Sar,Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala,d-aminolevulinic acid, cefadroxil and Ala-4-nitroanilide(all 100 lm, Table 1). Glycine, ... JC, Fujita T, Liang R, Ganapathy V & Lei-bach FH (1998) Identiﬁcation of a potential substratebinding domain in the mammalian peptide transportersPEPT1 and PEPT2 using PEPT1-PEPT2 and PEPT2-PEPT1 ... containing aromatic amino acids(24–34% trans content) obtained in a previous study of our group [23].To determine the stability of Bip-Pro, buffer sampleswere analyzed after incubating Caco-2...

báo cáo khoa học: " Identification and characterization of the Nonrace specific Disease Resistance 1 (NDR1) orthologous protein in coffee" doc

... ATGGTG CAA CAG CAG AAC CAA GAA A- 3’. The primersused for obtaining the single HA-tagged version wereCaNDR1-Forward 4 and CaNDR1-Reverse 2 (5’ -CTACAA CAG CAG AAC CAA GA-3’). PCR fragments ... itcan cleave RIN4, releasing RPS2 and initiating a diseaseresistance signaling pathway [25]. In this study, we cloned two C. arabica candidatecDNAs for NDR1 andanalyzedthededucedprimaryamino-acid ... were taken 7 daysafter inoculation. (b) and (c) Bacterial growth was monitored in planta by assaying leaf samples 0, 2, and 4 days post-inoculation. CaNDR 1a- expressing lines (T3-1, T3-2 and T3-3),...

báo cáo khoa học: " Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing" ppsx

... Omura Y, Abe K,Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K,Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M,WatanabeM, Hiraoka S, Chiba Y, Ishida S, Ono ... Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M,Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y,Nakamura Y, ... Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M,Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M,Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura...

báo cáo khoa học: " Identification and characterization of flowering genes in kiwifruit: sequence conservation and role in kiwifruit flower development" ppt

... in norma l and aberrant flowers. R eal-time RT-PCR analysis of the Actinidiaflowering genes in the leaf and floral organs of A. deliciosa ’Hayward’ (female, normal), ‘Chieftain’ (male, normal) ... McNeilage for help with the A. deliciosa’Pukekohe dwarf’ mutant, Lekha Sreekantan for involvement in early stages of the project, Andrew Gleave, Sakuntala Karunairetnam and Bhawana Nainfor cloning ... of pubescent bud scales in A. chinensis ’Hort1 6A and A. deliciosa ’Hayward’, respectively. Green arrow and arrowhead indicate leaf emergence in A. chinensis ’Hort1 6A and A. deliciosa ‘Hayward’,...

báo cáo khoa học: " Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase" ppsx

... kinase domain of AtHaspin, human Haspin, and fission yeast Haspin. Missing residues are shown as dashes, identical amino acids are shaded in gray, and residues of ATP/Mg2+ion-binding sites are shown ... aurora-like kinases in Arabidopsis and other plants. Plant Cell 2005, 17(3):836-848.11. Kawabe A, Matsunaga S, Nakagawa K, Kurihara D, Yoneda A, Hasezawa S,Uchiyama S, Fukui K: Characterization ... ied a Haspin kinase, designated AtHaspin, in Arabidopsis thaliana. The purified AtHaspinphosphorylated histone H3 at both Thr3 and Thr11 in vitro. Live imaging of AtHaspin-tdTomato and GFP -a- tubulinin...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... GTT GGA ATT CCA TCA TCA TCATCA TCA TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAGGAG GGA TAT GGG GAA C. The PCR reaction wasdone as described above. The ampliﬁed ... tyrosinase was alsoinactivated completely within 20 min [40]. In general,mammalian and plant-derived tyrosinases are not verythermostable; even a short incubation at 70–90 °C inac-tivates ... Theoretical study of phenol and 2-aminophenoldocking at a model of tyrosinase active site. New JChem 27, 909–913.62 Toussaint O & and Lerch K (1987) Catalytic oxidation of 2-aminophenols and...

Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulﬁde reductase from Pyrococcus horikoshii Implications for the disulﬁde metabolism of anaerobic hyperthermophiles doc

... (5¢-GGCCTCATGAAGAAAAAGGTCGTCATAATT-3¢), and TG101 (5¢-GGCCAAGCTTCTAGAACTTGAGAACCCTAGC-3¢) (for the P. horikoshii CoADR), and TG104 (5¢-CGCGCCATGGAAAAGAAAAAGGTAGTCATAA-3¢) and TG105 (5¢-CGCGGTCGACCTAGAACTTCAAAACCCTGGC-3¢) ... with a role forCoA in maintaining a reducing environment or servingas a pool of reducing equivalents at the very high tem-peratures and high concentrations of metals found in the natural environment ... theratio of protein concentration to ﬂavin concentration(as determined at 460 nm). blast and tfasta analysis of the phCoADR revealed a signiﬁcant level of identityto putative NADH oxidases...

Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx

... (5¢-GCGCGGGATCCTCATTTAAGCATGAAAACAACTTTGCC, antisense), containing NcoI and BamHI sites (underlined in the sequences). In order tointroduce an NcoI restriction site, an extra alanine codon(GCA) was introduced ... preculture was used toinoculate 1 L Luria–Bertani medium with kanamycin and spectinomycin (both 50 mgÆL)1) in a 2-L conical ﬂask and incubated in a rotary shaker at 37 °C until a cell density of A 600¼ ... effect of several salts, metals and inhibitors on theinitial activity of Pf-TDH was checked using butane-2,3-diol as substrate in the standard oxidation reaction and acetoin in the reduction reaction....

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... Nagasawa T, Ohkishi H, Kawakami B, Yamano H,Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-ala-nine chloride-lyase (deaminating) of Pseudomonas putidaCR 1–1. Puriﬁcation and characterization ... of the b-familycatalyse mainly b-replacement or b-elimination reac-tions. The d-alanine aminotransferase and the alanineracemase family are the other two independent lineages[6]. The b-family ... Isolation and characterization of a D-cysteinedesulfhydrase protein from Arabidopsis thalianaAnja Riemenschneider, Rosalina Wegele, Ahlert Schmidt and Jutta PapenbrockInstitute for Botany,...

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