Báo cáo khoa học: "Molecular characterization of two hantavirus strains from different rattus species in Singapore" ppsx

... screening of theanimals using PCR, and the characterization of two genetically different hantavirus strains denoted Seoulvirus strain Singapore and Serang virus strain JurongTJK/06 of samples from ... 7:15http://www.virologyj.com/content/7/1/15Page 8 of 9RESEA R C H Open AccessMolecular characterization of two hantavirus strains from different rattus species in SingaporePatrik Johansson1, Grace Yap2, ... S and M segments established that two different hantavirus strains circulate in the rodent population of Singapore. Notably, the hantavirus strains found in Rattus norvegicus clusters with other...

Báo cáo khoa học: Molecular characterization of artemin and ferritin from Artemia franciscana pot

... ferritin and the positioning of cysteine residues in artemin. A monomer of human H ferritin was depicted in Cn3D in theso called Ôneighbor styleÕ and used as a template for positioning of cysteine ... underlining indicateshelical regions, and residues in blue indicate b structures in Artemiaferritin and artemin. Residues in the di-iron ferroxidase centers of humanferritinHchainandinArtemia ... correspondingregion in ferritin has one. Artemin cysteines, with a singleexception, cluster at the ends of helices forming two intramolecular regions enriched in these residues, some of which...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... thebinding of RNA-binding proteins to 3¢ UTR tran-scripts has been poorly described in plants.PUF proteins are a large family of RNA-bindingproteins found in all eukaryotes. These proteins ... cell maintenance [7,15]. However, these two proteins show distinct patterning functions in the distalgerm line, independently affecting the number of cells in the mitotic region [29]. Also in C. ... FEBSBinding of APUM to NRETo test the predictions regarding the RNA-bindingspeciﬁcities of the putative APUM proteins, we investi-gated the capacity of APUM to bind to the NREsequence of hunchback...

Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... pro-tein–protein interaction. In this study, Osh6p wasdemonstrated to bind a pool of phosphatidylinositolphosphates (PtdInsP) including PtdIns(4)P, PtdIns(5)P,PtdIns(3,4)P2and PtdIns(3,5)P2with ... Faculty of Medicine, National University of Singapore, Republic of Singapore2 Institute of Molecular and Cell Biology, A*STAR Biomedical Research Institutes, Singapore, Republic of Singapore3 Institute ... staining in- creased, and ﬁnally punctate staining was almost lostwith predominant vacuolar staining at 30 min. Com-pared with wildtype, no delay of FM4-64 transport wasobserved in osh6D. In...

Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus potx

... mighthave remained in the Nox assay. The decrease in NADH in the ﬁrst assay was related to the amount of H2O2found in the second assay.Protein was determined according to Bradford [18] usingthe ... 3,3¢-dimethoxybenzidine as electron donor. In the NoxA-1 assay between 71% and 95% of the amountFig. 3. SDS/PAGE of extracts of recombinant E. coli containingNoxA-1, NoxB-1 or NoxC from A. fulgidus. ... protein assay kit, with BSA as standard.Analysis of catalytic propertiesKinetic parameters of NoxA-1 and NoxB-1 were deter-mined in the speciﬁc assay systems, by measuring the initialrate at different...

Báo cáo khoa học: Molecular characterization of a novel nuclear transglutaminase that is expressed during starﬁsh embryogenesis ppt

... : 26 of the original volume using Centricon-10 (Amicon).Proteins were determined by the modiﬁed method of alkaline copper (Lowry) protein assay [23] using BSA as thestandard.Transglutaminase ... localization of TG has been reported in the studies on tissue TG [8,9]. Anuclear transport protein, importin-a3, has been shown tobe involved in the active transport of tissue TG into thenucleus in ... protein produced in an oocyte bymicroinjection of pHE-TG, which c ontains the Drosophilaheat shock protein promoter and a gene that encodes thefusion protein (Fig. 8A), into the germinal vesicle,...

Báo cáo khoa học: Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis docx

... (1999) Vaccination with cathepsin L proteinasesand with leucine aminopeptidase induces high levels of protection against fascioliasis in sheep. Infect Immun 67,1954–1961.3 Williamson AL, Brindley ... modeling of substrate binding in the S1¢ subsite of serine carboxy-peptidases from yeast, wheat, and human. Biochemistry35, 14899–14909.23 Pshezhetsky AV, Vinogradova MV, Elsliger MA, el-Zein ... molecular cloning andbiochemical characterization of two inducible chymo-trypsins. Eur J Biochem 268, 4027–4035.33 Akov S (1982) Blood digestion in ticks. In Physiology of Ticks (Obenchain FD &...

Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx

... proteinkinases tested in this study represent most of the p rincipalclasses of protein serine/threonine kinases, the possibilityremains that an untested, perhaps unidentiﬁed, proteinkinase ... omeproperties of 5¢-nucleotidase, adenosine kinase and adenosinedeaminase in tissues of vertebrates and invertebrates in relation tothe control of the concentration and the physiological role of adenosine. ... for protein puriﬁcation.Keywords: adenosine kinase; adenosine r egulation; proteinserine/threonine kinases; CNS.Adenosine (Ado) is a potent biological mediator and a keyparticipant in cellular...

Báo cáo khoa học: Molecular characterization of secretory proteins Rv3619c and Rv3620c fromMycobacterium tuberculosisH37Rv docx

... enhancements of ﬂuorescenceintensity, suggesting the relocation of tryptophans in thehydrophobic environment because of membrane bind-ing. We also checked the binding of the two proteins tosmall ... Rv3020ccontains histidine in place of tryptophan in the WXGmotif, which induces the formation of a small helixthat joins the N-terminal and C-terminal domains [13].The ESAT-6 ⁄ CFP-10 homologs in Staphylococcusaureus ... Rv0287–Rv0288 could result from the dif-ference in the folding state of Rv3620c from that of CFP-10 and Rv0287.Thermal unfolding of proteinsThe presence of any stable tertiary structure in Rv3619c and...

Báo cáo khoa học: Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference pptx

... primers used in 3¢-RACE of Pem-GIHcDNA were designed from the conserved amino acidsequences of MIH ⁄ GIH from several species of crustacean. In the first round of PCR, a 1 lL aliquot of cDNA wasamplified ... compilation ª 2008 FEBSMolecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interferenceSupattra Treerattrakool1, ... auto-mated DNA sequencing.The recombinant plasmid of hairpin-RNA of Pem-GIHwas subsequently transformed into an RNaseIII-deficientE. coli HT115 strain. Expression of hairpin-RNA wasinduced with 0.4...

Xem thêm