... protease inhibitors (5 lgÆmL)1leupeptin,2 lgÆmL)1aprotinin, 80 ngÆmL)1pepstatin A). Briefly, cellswere lysed in ice-cold hypotonic buffer (1 mMTris/HCl,pH 7.4) for 30 min at 4 °C. Following ... tetraethylammo-nium chloride, Triton X-100, Tween 20, verapamil and vincristine were all obtained from Sigma Chemicals. Calceinwas purchased from Molecular Probes. Staurosporine and bisindolylmaleimide ... clotrimazole, creatine kinase, creatinephosphokinase, daunorubicin, dideoxyforskolin, iso-butylmethylxanthine (IBMX), doxorubicin, estradiol 17-b-D-glucuronide, forskolin, glibenclamide, glutathione(reduced...