báo cáo khoa học: " Functional mapping of reaction norms to multiple environmental signals through nonparametric covariance estimation" ppsx
báo cáo khoa học: " Functional mapping of reaction norms to multiple environmental signals through nonparametric covariance estimation" ppsx
... 11:23http://www.biomedcentral.com/1471-2229/11/23Page 9 of 13METH O D O LOG Y AR T I C LE Open Access Functional mapping of reaction norms to multiple environmental signals through nonparametric covariance estimationJohn ... of different environmental factors.Results: We implement a more robust nonparametric covariance estimator to model these interactions within theframework of functional mapping of reaction norms ... et al.: Functional mapping of reaction norms to multiple environmental signals through nonparametric covariance estimation. BMC Plant Biology 2011 11:23.Submit your next manuscript to BioMed...
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Báo cáo khoa học: Functional implications of pigments bound to a cyanobacterial cytochrome b6f complex potx
... rate of 0.7 mLÆmin)1. (A) Acetoneextract of purified cyt b6f of the PS1-less mutant. (B) Absorbancespectrum of echinenone. (C) Acetone extract of purified cyt b6f of the PS1-less ⁄ CrtO-less ... the absorption spectrum of chloro-phyll and the redox state of a b-type cytochrome. To determine the orientations of the various cofac-tors with respect to the long axis of the cyt b6f particle,linear ... Con-figuration of the carotenoid in the reaction center of photosynthetic bacteria. 2. Comparison of the Reso-nance Raman lines of the reaction center with those of the 14 different cis-trans isomers of...
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... that, according to prior studies of nonhemeFe2+centers [29–31], is assigned to the mixing of the4p orbital with the 3d orbital of the metal cofactor.Two important pieces of information can ... number of the Fe2+center [29–31]. The value of (12 ± 1) · 10)2eV there-fore indicates that the Fe2+bound to Bxe_A2876 iscoordinated by a total of five ligand atoms.An initial estimation of ... formation of monoacetylacetoatoiron(III)and the effect of copper (II) on the formation of monothenoyltrifluoroacetonatoiron (III). Inorg Chem10, 2096–2101.34 Straganz GD, Hofer H, Steiner W &...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... correct position to initiate the catalytic reaction. The transition state of this type of kinases isconsidered to be close to trigonal bipyramidal geo-metry of the phosphate to be transferred. ... mutagenesis of four activesite residues of Dm-dNK. Residue E52 was mutated to D, H and Q, residue Y70 to W, residue Q81 to N andresidue R105 to K and H. The kinetic properties of the active ... consequence of the tryptophan creatingsteric hindrance for the purines. The intention behindthe mutation of Y70 to W was to create an enzyme withincreased affinity towards ACV, because tryptophanwas...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... interaction of DEC1 with BMAL1 has been suggested a s one of themolecular mechanisms of the suppression [Honma, S.,Kawamoto, T., Takagi, Y., Fujimoto, K., Sato, F.,Noshiro, M., Kato, Y. & ... 10 ng) of FLAG-DEC1 expression vector were co-transfected with pDEC1-E-ABC-TK, together with expression vectors for CL OCK and BMAL1. The total amount of transfected DNA wasadjustedtothesamevalue,ineachexperiment,bytheadditionofanemptyvector ... CLOCK/BMAL1 through com-petition for binding to E-boxes and/or protein–proteininteractions of DECs with BMAL1 [ 7]. Furthermore, w erecently demonstrated the existence of a novel autofeedbackloop...
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Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in fibrinolysis and plasmin-induced cell detachment and apoptosis docx
... and to a hyperbolic decay equationthat allows calculation of the concentration of mAb thatproduces 50% of the inhibitory effect (IC50) [61].Effect of anti-LBS mAbs on the activation of plasminogen ... microscope. The apoptotic index was calculated asthe percentage of TUNEL-positive nuclei relative to totalDAPI-stained nuclei.Effect of anti-LBS mAbs on fibrinolysisThe activation of 50 nm of either ... Blockage by mAb A10.2 of K4-LBS exposedupon Glu-Pg binding to surfaces, may also contribute to this inhibitory effect.We then investigated the consequence of the inhibi-tion of plasminogen binding...
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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx
... theirability to bind to the junction in vitro. The truncations didnot prevent the localization of Ydc2 to mitochondria butthe mutants showed no activity in vivo. Significantly, the loss of Ydc2 led to ... Subcellular localization of Ydc2 and truncated mutants to yeast mitochondria. Fluorescence micrographs of Ydc2–EGFP fusions in ydc2DS. pombe cells (green), MitoTracker staining of mitochondria (red) ... truncated Ydc2 mutant proteins localize to the mitochondria inS. pombeYdc2 (30.2 kDa) fused to the ORF of GFP was shown to localize in the mitochondria of S. pombe [16]. Scanning thefull-length...
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Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx
... thedimerization of EF-Ts in the crystal. In the crystal of E. coliEF-Tu–EF-Ts, a quaternary complex, formed by twomolecules of each of the elongation factors, is observed.The coiled-coil motifs of each of ... is 78% of the point of growthinhibition of UY211 (D420¼ 0.60). The point of growthinhibition of GRd.tsf when expressed as a percentage of that of UY211, was independent of the initial proline ... both groups of media.Therefore, the growth rate of GRd.tsf ranges from 70–95% of that of UY211 (Fig. 3). The lowest growth rate of GRd.tsf (70%), relative to the growth rate of UY211, wasobtained...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... measured in the presence of acti-vators (pyruvate or PEP) and inhibitors (NADH orATP) (Fig. 5A). The results indicate that PEP is able to reverse, in part, the inhibitory effects of bothNADH and ATP ... (Fig. 5A). In the case of pyruvate,although partial reversal of NADH inhibition wasobserved, total reversal of ATP inhibition was found(Fig. 5A).The regulatory properties of the truncated Ptas ... chromatography,validating the use of this technique for estimating thenative assembly of this protein.On the other hand, in order to evaluate the contri-bution of the N-terminal end to the formation of thefinal...
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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
... with the binding of 15E11 to the intact repeat region (Fig. 6C,D). Theresidual binding of 15E11 to FnBRAD9,10 could be aconsequence of the weak binding of repeats 4 and 5 to the antibody.15E11 ... 3. SPR analysis of NTD binding to FnBRs of FnBPA and FnBPB fused to GST. Different concentrations of the NTD were compared forbinding to GST–repeats immobilized on the surface of a CM5 sensor ... FnBRs of FnBPBA panel of mouse mAbs was produced against therecombinant repetitive region of FnBPB. Analysis of mAbs binding to the recombinant FnBR indicated thegeneration of two categories of...
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