báo cáo khoa học: " Patterns of sequence polymorphism in the fleshless berry locus in cultivated and wild Vitis vinifera accessions" pdf

... for the 69 genome fragments at the top of chromosome 18 in the cultivated and the wild compartmen t of Vitis vinifera and details of the shared and specific insertions/deletions(INDELs) and ... from the flb region in wild and cultivated grapevines. Venn diagram showing the distribution of the 554non-redundant SNPs found in the 948 kb region at the top of chromosome 18 in the sets of wild ... parametersobtained in the cultivated and the wild pools of Vitis vinifera. Additional file 4: supplemental table S4. qPCR validation of homozygosity in the flb region in Ugni Blanc mutant. Estimation of the...

Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

... to the a-globin gene locus dur-ing the differentiation of erythroid cells. The regula-tory elements located upstream of the a-globin genesalso appear to initiate the activation of the gene locus. However, ... gene promoters and to the LCR of the murine b-globin gene locus in MEL cells. (A) Schematic repre-sentation of the murine b-globin gene locus. LCR hypersensitive sites and globin genes are shown ... subsets of genes. The sequential silencing of genes involved in maintenance of pluripotent and multipotent states and the activa-tion of those involved in differentiation is believed tobe a dominant...

Tài liệu Báo cáo khoa học: Effect of sequence polymorphism and drug resistance on two HIV-1 Gag processing sites pdf

... constants with the wild- type PR(Table 3). The mutated residues in this sequence are in the outer regions of the cleavage site sequence recognized by the PR, and the respective S4 and S3 binding sites ... 2).Increasing the concentration of the NC/p1 substrate above the Kmresulted in a decreased velocity, suggesting the possibility of increased nonproductive binding at the substrate binding site. Nevertheless, ... increases with the time of therapy. Schock et al. [6] proposed thatmutations located in the binding cleft of the enzyme canTable 4. Processing of peptides representing wild- type and mutant HIV-1...

Báo cáo khoa học: Characterization of sequence variations in human histone H1.2 and H1.4 subtypes pptx

... conﬁrm the H1.2 Ala17Val sequence variation, a183 bp PCR product was ampliﬁed in the 5¢-UTR, and the start of the coding sequence of the H1.2 gene cor-responding to the N-terminal tail of the ... tail and the binding of H1.4 to chromatin,as arginine offers additional hydrogen-bonding abilitiesto DNA as compared to lysine. Lysine 173 in H1.4 issituated in an SPKK motif, one of the known ... codon 18 in the H1.2 gene. Therewere remains of the wild- type C at g578 (Fig. 4),explaining the minor wild- type H1.2 peak in the HILIC chromatogram. This result is probablyexplained by the nondiploid...

báo cáo khoa học: " Patterns of research utilization on patient care units" pps

... Pitts N: Changing the behavior of healthcare professionals: The use of theory in promoting the uptake of research findings. J Clin Epidemiol2005, 58:107-12.4. The Improved Clinical Effectiveness ... participated in conceptualiza-tion of study, securing grant funding, in the start-up of the study (with data collection) and served as a lead investiga-tor for the adult study, coordinating one of the ... conceptu-alization of study, securing grant funding, in the start-up of the study (with data collection) and served as a leadinvestigator for the pediatric study, coordinating one of the participating sites,...

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... intensivecare unit during the studies noted that interns in the intervention schedule “often knew very little about the patients who had been admitted the night before” and that the “intern coming on at ... errors.”Differences in the incidence of serious errors were analyzedusing rates (per patient-day), and therefore the fact that therewere more patient-days in the traditional schedule cannotexplain the results. ... increases after 16 hours of sustainedwakefulness, a finding reflected in the twofold increase in interns’ attentional failures after they had been working formore than 16 hours on the traditional schedule...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a coupled oscillator-based mechanism in smooth muscle ppt

... typically in the range 2–3 mm [26].Finding experimental evidence that electrical cou-pling is the key ‘spring’ interlinking the Ca2+storeshas involved repeating the decoupling experiment of Fig. ... composed of a large number of Ca2+store oscillators. In this simulation, the intrinsic frequencies of oscilla-tors are different from each other, and as the [Ins(1,4,5)P3] is increased in the ... asshown in the study of Yamashita [36] (see accompany-ing review), and it will be interesting to determine the relative role of this mechanism. In summary, store-based pacemaking, whether oper-ated...

Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc

... determined. Finally, we assignan important role to the endosomal acidic cathep-sins B and D in increasing the in vitro transfer of the ADP-ribosyl moiety of NAD+to EF-2 by ETA,but not in the ... revealed the N-terminal sequence of ETA(AEEAFDL), suggesting that the cleavage sites arelocated within the C-terminal region of the toxin.N-terminal sequence analysis of ETA fragments 6b and 6d, ... internal-ized toxin, based on the following: (a) the lack of sensitivity of endosomal ETA-degrading activity tofurin inhibitors [hexa-d-arginine (HA)]; and (b) the predominant association of low molecular...

Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt

... was preincubated for 10 min and assayed in the presence of the given concentrations of the hydro-phobic probe bis-ANS, using TO as substrate. The activity of phos-phorylated HSL in the TO assay ... exposes and ⁄ or increases the lipid-binding area of the enzyme. A direct demonstration of this presumedconformational change will have to await the solving of the atomic structure of HSL in its ... phospholipids and side chains of particularamino acids, thus accounting for the increased capacityto penetrate to the interior of the vesicle. Phospholipidvesicles mimic the lipid droplets found in vivo,...

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... vitroactivity of acyl-CoA:ergosterol acyltransferase wasdetermined in an are1 deletion background. The enzyme activity in the ste20D strain and the cla4D strainwas indistinguishable from that in the wild- type ... lethal, indicating that CLA4 and these genes, encod-ing proteins involved in sterol synthesis, may function in the same pathway(s) [25]. It is not clear how Cla4pcould in uence sterol synthesis. In ... 7263rapid increase in sterol synthesis, but rather clustering of existing sterol molecules and ⁄ or a highly focusedtransport towards the tip of mating projections. In this work, we also examined the...

Xem thêm