... The influence of Mg2+on the kinetic properties of APase from E. coli. The influence of Mg2+on the kinetic properties of APase from E. coli inthepresenceofaZn2+to dimer ratio of 2 : 1 in 2A2M1Pbuffer, ... structure and the reaction mechanism of APase from E. coli, suggested by Kim and Wyckoff [1], aswell as the high resolution crystal structure determined byStec et al. [10], offers clarification of ... form of a dianion [9],however, the slow dissociation of Pi,andtheslowphosphorylation of Ser102 by Pi, are both in accordance with Pibinding in form of a trianion.The crystal structure of...