báo cáo khoa học: " Production of viable male unreduced gametes in Brassica interspecific hybrids is genotype specific and stimulated by cold temperatures" potx

... combinations in the production of unreduced gametes in Brassica interspecific hybrids, and somehybrid genotypes were stimulated by cold temperaturesto produce high levels of unreduced gametes. ... 67:367-375.doi:10.1186/1471-2229-11-103Cite this article as: Mason et al.: Production of viable male unreduced gametes in Brassica interspecific hybrids is genotype specific and stimulated by cold temperatures. BMC ... which is useful for assessing the production of unreduced gametes. Unreduced gametes have been observed in a number of Brassica interspecific hybrid types [14-18] including hybrids of the Brassica...

báo cáo khoa học: "Production of viable hybrids in salmonids by triploidization" ppt

... permitted by artificial insemi-nation in Tilapia : Induced diploid gynogenesis, production of all-triploid populations and intergencric hybridization. Proceedings of the International ... However,Electrophoregrams of MDH and muscle proteins are given in figures 4 and 6 and their interpretation in figures 5 and 7, respectively :- Cathodal muscle proteins and PGI were ... leading to acomplete elimination at the end of P4....

Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf

... obtained by Bio-Gel P-4 puriﬁcation of the included volume of the column in (A), largeO-glycans (j), obtained as the included volume of the column in (C), and small O-glycans (s), obtained ... with a linearized plasmid containing a 553-bpfragment of b3Gal-T5 cDNA, that includes the initial360 bp of the coding sequence and 192 bp of the 5¢untranslated region of the gene, placed in the ... bothantigens is prevented by benzyl-a-GalNAc, an inhibitor of O-glycosylation, while it is not affected by swainso-nine, an inhibitor of N-glycosylation. These resultsconﬁrm that b3Gal-T5 is responsible...

Báo cáo khoa học: Downregulation of protease-activated receptor-1 in human lung ﬁbroblasts is speciﬁcally mediated by the prostaglandin E2 receptor EP2 through cAMP elevation and protein kinase A pot

... of PAR-1 after 7 h of treatment of hLFs with PGE2, forskolin, butaprost, ISO, and NECA.Surprisingly, ISO and NECA appeared to be lesspotent than PGE2 and the other cAMP-inducingagents in ... Using a speciﬁc acti-vator of Epac, 8-CPT-2¢-O-Me-cAMP, we have shownthat Epac is not involved in downregulation of mRNA and protein levels of PAR-1. On the other hand, inhi-bition of PKA by ... mediated by theprostaglandin E2receptor EP2 through cAMP elevation and protein kinase AElena Sokolova1, Roland Hartig2 and Georg Reiser11 Institut fu¨r Neurobiochemie, Medizinische Fakulta¨t,...

Báo cáo khoa học: "Lack of Cetuximab induced skin toxicity in a previously irradiated field: case report and review of the literature" pdf

... [4]. Inhibiting the EGFR - by directly blocking theextracellular EGFR receptor domain with monoclonalantibodies or by inhibiting the intra-cytoplasmic ATPbinding site with tyrosine kinase inhibitors ... of the Effect of Radiation on Erlotinib-Induced Skin Rash. J Clin Oncol 2007, 25(15):2140.19. Gerber PA, Enderlein E, Homey B, et al.: Radiation-induced prevention of erlotinib-induced skin ... parotid gland. At this point, he wasdeemed unresectable and was offered palliative chemo-therapy.DiscussionEGFR is expressed in undifferentiated, proliferating kera-tinocytes in the basal and...

Tài liệu Báo cáo khoa học: Production of biologically active forms of recombinant hepcidin, the iron-regulatory hormone docx

... absorption and serum iron levels by inhibiting dietary intestinal iron absorption and iron recycling by the macrophages [18]. This is achieved by hepcidin binding to ferroportin, the trans-membrane iron ... secreted and acts by controlling the concentration of the membrane iron exporterferroportin on intestinal enterocytes and macrophages. Hepcidin binds toferroportin, inducing its internalization and ... areduction of hepcidin secretion whereas, in contrast,overexpression of hepcidin appears to play a deter-mining role in anemia of in ammation or in ammation of chronic disease [13] and iron-refractory...

Báo cáo khoa học: Production of a recombinant mouse monoclonal antibody in transgenic silkworm cocoons pptx

... and H-chain genes to obtain silkwormsbearing ie1 and the L- and H-chain genes.Analysis of recombinant proteins in cocoonsCocoon fragments were suspended in an extraction buffercomprised of ... deter-mination. Protein G, as well as protein A, binds theCH2 and CH3domain interface region distal to theglycosylation site in the CH2domain of IgG [29], and the afﬁnity of the IgG-binding ... amount of mAb in thesericin layers were calculated by quantifying the bandintensities of the CBB-stained H-chains. When theextracted proteins with NaCl ⁄ Piwere analyzed, faintbands of the...

Báo cáo khoa học: "Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf)" ppt

... ...

Báo cáo khoa học: "Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes" pot

... monolayer of cells had formed. A suspension of single cells was prepared by trypsinization of the cultured cells, followed by resuspension in TLH containing 0.4% (w/v) bovine serum albumin (TLH-BSA) ... University of Agriculture and Veterinary Medicine, Japan, and conducted in accordance with the Guiding Principles for the Care and Use of Research Animals.Culture mediaUnless otherwise stated, ... containing 0.05% (w/v) polyvinyl alcohol (TLH-PVA) [2] and then washed once in IVM medium. Groups of 70-80 COCs were placed into the wells of a four-well multidish (Nunc, Denmark) containing...

Báo cáo khoa học: " Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences" docx

... characteristics. AAV is capable of infecting dividing and non-dividing cells in vitro and in vivo and of infecting cells originating from multiple spe-cies and tissue types. No human disease has ... are involved in determin-ing AAV2 virus formation. In the present studies, a mutation from ATG to ACG in thestart codon decreased Rep78/68 protein. This finding is consistent with that of Li ... proliferation and differentia-tion across a wide spectrum of cell types. Acting by bothendocrine and paracrine/autocrine mechanisms, it plays acentral role in the development and maintenance of mul-tiple...

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