Báo cáo y học: "Kinetics and isotype profile of antibody responses in rhesus macaques induced following vaccination with HPV 6, 11, 16 and 18 L1-virus-like particles formulated with or without Merck aluminum adjuvant" ppt

... of Immune Based Therapies and VaccinesOpen AccessOriginal researchKinetics and isotype profile of antibody responses in rhesus macaques induced following vaccination with HPV 6, 11, 16 and ... Immunoassay (cLIA). Sera from rhesus macaques immunized with 2 µg each of HPV 6, 11, 16 and 18L1-VLPs formulated with Merck Aluminum Adjuvant (MAA) (-◆-) or 2 µg each of HPV 6, 11, 16 and 18L1-VLPs ... quadrivalent vaccine,composed of HPV 6, 11, 16, and 18 L1 VLPs formulated with MAA. This vaccine has also been shown to be effec-tive in preventing persistent infection and HPV 6, 11, 16 and 18 related...

Báo cáo y học: "The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia" pdf

... content of the papain digest was determined by measuring Hoechst33258 dye (Sigma) binding with a Hoeffer Dyna Quant 200fluorometer. Glycosaminoglycans were assayed in the papaindigest by using ... [22] of meniscus cells in vitro and also to maintain the ability of mon-olayer expanded chondrocytes to redifferentiate [ 16, 2 3].Materials and methodsCell isolation and expansion With informed ... similar in monolayer and cell aggregates, but biglycan wasincreased by low oxygen tension in cell aggregates formedfrom FGF2-expanded cells. Histology showed an increase in safranin-O staining in...

Báo cáo y học: "Response to: Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection – authors’ reply" ppt

... (ACS) in this study was prepared in a GMP (good manufacturing practice) facility in strict accordance with the guidelines supplied by the manufacturer (Orthogen, Düsseldorf, Germany) and was injected ... to determine the cytokine levels before and during ACS treatment. We showed that, 3 days after injection, cytokine levels were at baseline and had no secondary eﬀ ects on other cyto kines.  ... 3-day intervals in accordance with the instructions given. As recom-mended, immediately before injection of ACS, synovial ﬂ uid was carefully aspirated to minimize ACS dilution.  is synovial...

Báo cáo y học: "The gene expression profile of preclinical autoimmune arthritis and its modulation by a tolerogenic disease-protective antigenic challeng" ppt

... been reported. Therefore, we undertook this important and timely study of the gene expression analysis in AA. In this study, we examined the gene expression profiles of the draining lymph node ... molecules (Tables 1 and 2). Not surprisingly, the Bhsp65 -induced gene expression profile mostly reinforced the immune-based and inflammatory nature of AA. The expression level of important arthritis-related ... expression of many pro-inflammatory genes most likely reflects the attempt of the host to counter the emerging inflammation. The infiltration of inflammatory cells into the joints is believed to initiate...

Báo cáo y học: "An open pilot study of zonisamide augmentation in major depressive patients not responding to a low dose trial with duloxetine: preliminary results on tolerability and clinical effects" ppsx

... thuscontinuing the study with zonisamide 75 mg/day once aday augmentation and maintaining duloxetine 60 mg/day once a day. By definition, week 12 HAM-D t otalscores were significantly lower ... neurotransmitters involved in the patho-physiology of depression, including dopamine and seroto-nin [11-13]. Therefore, in consideration of a partialpharmacodynamic overlap between lamotrigine and thelatterly ... commonly used anti-Parkinsonian drugs,significantly improved the primary symptoms of patients with advanced Parkinson’s disease, possibly by activatio n of dopamine synthesis, inhibition of monoamine...

Báo cáo y học: "Magnetic resonance imaging changes of sacroiliac joints in patients with recent-onset inflammatory back pain: inter-reader reliability and prevalence of abnormalities" potx

... therefore be ascertained in future, by following thepatients longitudinally and obtaining more data, which willoccur in this cohort. The development and choice of an appro-priate scoring system ... reported a history of both uveitis and IBD, and one patient reported psoriasis and IBD. Of the 25 patients(37%) with a family history of AS, four patients had a medicalhistory of uveitis and ... pain present (%) 96aHLA-B27 present (%) 46History of inflammatory bowel disease present (%) 15History of uveitis present (%) 15History of psoriasis present (%) 24Family history of ankylosing...

Báo cáo y học: "Four-year follow-up of infliximab therapy in rheumatoid arthritis patients with long-standing refractory disease: attrition and long-term evolution of disease activity" pot

... respectively, of predicting withdrawal of infliximabtherapy due to inefficacy (Table 3). Taking into account the13.6% a priori chance to withdraw therapy due to inefficacy, aDAS28 of at least ... 511patients with long-standing refractory rheumatoid arthritistreated with intravenous infusions of infliximab 3 mg/kg at weeks0, 2, 6, and 14 and every 8 weeks thereafter for 4 years. Amongthe initial ... values) [16] .Survival analysis and predictors of continuation of infliximabtherapy were analysed by means of Kaplan-Meier analysis and Cox regression. Cox regression analysis was performed with the...

Báo cáo y học: "RANKL increases the level of Mcl-1 in osteoclasts and reduces bisphosphonate-induced osteoclast apoptosis in vitro" pps

... rapidly in mouse osteoclasts following cytokine starvation but are restored by pro-survival factorsMcl-1 levels decrease rapidly in mouse osteoclasts following cytokine starvation but are restored ... clodronate or alendronate for up to 48 hours in the absence or presence of RANKL. Changes in cellmorphology and induction of apoptosis were examined byscanning electron microscopy, whilst resorptive ... RANKL (and other pro-inflammatory factors suchas TNF- and LPS) to increase the level of Mcl-1 in osteoclastsmay explain the lack of effectiveness of some bisphosphonates in preventing inflammation-induced...

Báo cáo y học: " Phase IIb randomized trial of adjunct immunotherapy in patients with first-diagnosed tuberculosis, relapsed and multi-drug-resistant (MDR) TB" doc

... which following chemical- and heat inactivation was formulated into an oral pillaccording to proprietary technology developed by us [6].It is well known that one third of people carry M. tuber-culosis ... of revaccination with BCG in early childhood onmortality: randomised trial in Guinea-Bissau. BMJ 2010, 340:c671.32. Silin DS, Lyubomska OV, Jirathitikal V, Bourinbaiar AS: Oral vaccination: where ... candidates are in the first category and onlyM. vaccae and RUTI are considered to be in the secondcategory [35]. Studying V5 as post-exposure vaccine cancontri bute to better understanding of th e...

Báo cáo y học: " The anti-inflammatory effects of the tellurium redox modulating compound, AS101, are associated with regulation of NFB signaling pathway and nitric oxide induction in macrophages" doc

... hibition of two steps in the NFBpathway by modifying specific cysteine residues in IKKa and in the p50-subunit resulting in the inhibition of nuclear translocation and DNA-binding to the iNOSpromoter. ... part of host defenses against microorgan-isms. Macrophages are the main proinflammatory cellsresponsible for invading pathogens by releasing proin-flammatory mediators such as IL -6, including ... themajority of the assays, performed the statistical analysis, participated in thedesign and coordination of the study, and drafted the manuscript.Figure 4 Inhibition of p50 DNA-binding in iNOS...

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