Báo cáo khoa hoc:" Formation and stability of oil-in-water nanoemulsions containing rice bran oil: in vitro and in vivo assessments" pdf

Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc

... denaturant–binding interactions;histidine-phosphocarrier; protein stability. A full understanding of the physical interactions underlyingthe structure, folding and the function of a protein requiresa ... borne in mind that the mostprobable origin of the discrepancies is the experimentaluncertainty in determining the third derivative of the stability curve (mDCpi), obtained, in most of the ... (enzymeI of the PTS), the ﬁrst protein in the cascade of proteinsforming the PTS, to HPr, the histidine-phosphocarrierprotein. HPr is the smallest protein in the protein cascade of the PTS and it...

Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea ﬂexuosa Comparison of twelve xylanases in relation to their thermal stability pdf

... thermophilic proteins contain morearginines on the protein surface than mesophilic proteins[36–38]. The effect of the large-scale increase in the number of arginines was tested experimentally in T. reesei ... in bleaching of pulp to reduce the use of toxic chlorine- containing chemicals [1] or to improve the quality of animalfeed [2]. In addition, there are applications in the food and beverage industry ... higher protein density and lower void volumesindicating better packing. In the comparison of PDB structures, Karshikoff and Ladenstein [51] have observed that proteins from thermo-philic and mesophilic...

Báo cáo khoa học: N-Terminal segment of potato virus X coat protein subunits is glycosylated and mediates formation of a bound water shell on the virion surface docx

... substitution of 11 serine and threonine residues with g lycine and alanine, whichwould increase the peptide hydrophobicity. The minoramounts of other peptides observed in the chromatographicproﬁles in ... proximity to theprotein surface is fundamental to protein f olding, stability, recognition and activity, and thus understanding proteinhydration is crucial in unraveling protein functions [31].Virus ... peptide;658 Da, potassium salt of the same peptide and/ or sodium salt of hexose -containing peptide; 674 Da, potassium salt of hexose -containing peptide;additional peaks in lower spectrum correspond...

Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc

... of insoluble substrates. Bound protein wasdetermined indirectly by measuring the concentration of freeprotein in the supernatants after 24 h of incubation.L. lactis chitinase and chitin-binding ... carbohydrate-binding proteins and domains from T. fusca [26].Genes encoding family 33 CBPs occur even in bacte-ria containing otherwise seemingly simple chitinolyticmachineries, such as in the lactic ... binding surface (Fig. 3B). Inter-estingly, the largest insert contains two aromatic aminoacids (Trp51 and Phe55), which could interact with thesurface of a chitin crystal. Another interesting...

Tài liệu Báo cáo khoa học: Efﬁcient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf

... distinct functions in cells. STAT3 is mostly involved in cell survival and proliferation, and STAT1 is involved in anti-viral and immune defence and cell death, in response to interfer-ons, including ... lg of nuclear protein extracts were incubated for 30 min at4 °C in binding buffer (1% NP40, 50 mm Hepes, pH 7.6,140 mm NaCl) containing salmon sperm DNA (1 lg perassay) and 1 lg of biotinylated ... hairpin decoy, but not control ODN, inhibitedcell proliferation, eliminating any possible effects as aresult of the introduction of DNA within cells, and indicating that, in itself, the interaction...

Báo cáo khoa học: A novel mechanism of TGFb-induced actin reorganization mediated by Smad proteins and Rho GTPases docx

... 24 h in the absence or presence of the general inhibitor of transcription actinomycin D, and changes in the actin cytoskeleton were monitoredby ﬂuorescence using rhodamine phalloidin staining. ... and titrated in HEK293 cells as described previously [22]. Adenoviraltransient infections of cells using multiplicity of infectionleading to a maximal rate of infection efﬁciency and protein ... [24,27–29 and unpublished data]. Having established that overexpres-sion of R-Smad proteins results in long-term actinpolymerization, we investigated the involvement of Smad2 and Smad3 in TGFb1-induced...

Báo cáo khoa học: "Low-cost Enrichment of Spanish WordNet with Automatically Translated Glosses: Combining General and Specialized Models" pdf

... Translation Models In this section we study the possibility of combin-ing out -of- domain and in- domain translation mod-els aiming at achieving a good balance betweenprecision and recall that yields ... second baseline system(‘WNG’) is entirely based on the training set from of the in- domain corpus of parallel glosses. In thesecond case phrase pairs occurring only once in the training corpus ... continue improving asmore in- domain data are added. We observe avery signiﬁcant increase by just adding around3,000 glosses. Another interesting observation isthe boosting effect of the combination...

Báo cáo khoa học: Engineering thermal stability of L-asparaginase by in vitro directed evolution ppt

... purposes.This incompatibility often relates to the stability of the enzymes under body conditions. In the case of engineering proteins for thermostability, researchersare in the enviable position of being ... glutamatedehydrogenase and sample containing l-ASNase activity.Alternatively, the rate of ammonia formation was measuredEngineering thermal stability of L-asparaginase G. A. Kotzia and N. E. Labrou1758 ... thermostability. Screen-ing of a library of random Asp133 mutants conﬁrmed that this position isindeed involved in thermostability and showed that the Asp133Leu muta-tion confers optimal thermostability.AbbreviationsEcaL-ASNase,...

Báo cáo khoa học: An NMR study of the interaction between the human copper(I) chaperone and the second and ﬁfth metal-binding domains of the Menkes protein pot

... metal-binding domains of ATP7A)protein–protein interaction between MNK2 and HAH1 is unlikely. Given that the surface charges of MNK2 and WND2 are fairly similar, a preferentialinteraction of the ... surface of Atx1 and HAH1 isquite similar, but that of the metal binding domains of Ccc2 is somewhat different from ATP7A ⁄ ATP7B[11]. In addition, although the two metal-bindingdomains of Ccc2 ... Bertini I, Cioﬁ-Baffoni S, Del Conte R &Gonnelli L (2003) Understanding copper trafﬁcking in bacteria: interaction between the copper transport pro-tein CopZ and the N-terminal domain of...

Báo cáo khoa học nông nghiệp " Improvement of Vietnamese vegetable production using GAP principles for seed and seedling production and superior hybrid varieties " MS3 doc

... was of a high standard, even for international seedling production. His knowledge and skills and record keeping, and those of his senior staff (including his wife) were of a very high standard, ... training in tomato and/ or cucumber production. They displayed good knowledge of the principles and practices of IPM and the major tomato and cucumber pests and diseases, but were less aware of ... outward-looking. SSC is a member of The Asia and Pacific Seed Association, and has links with international seed companies, including in Australia. They expressed a particular interest in both evaluating...

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