báo cáo khoa học: " Lessons from the evaluation of the UK''''''''s NHS R&D Implementation Methods Programme" doc
báo cáo khoa học: " Lessons from the evaluation of the UK''''s NHS R&D Implementation Methods Programme" doc
... the NHS of the applications submitted, on the progress of commissionedwork, and on the quality and value to the NHS of the IMPas it developed. There was some overlap in membershipbetween the ... care.DiscussionThis evaluation was designed to explore the quality of the outputs of the IMP and of the commissioning process.Our findings regarding the impact of the work funded andabout the difficulties ... re-assessment of the organisation of the NHS and consequent change is amajor impediment to the successful implementation of research in the NHS. In this respect nothing has altered. Ifanything the scale...
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Tài liệu Báo cáo khoa học: Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium doc
... transfer from CDH to cytochrome c, Phe166, which is located in the cytochromedomain and approaches one of propionates of heme, was mutated to Tyr,and the thermodynamic and kinetic properties of the ... the special features of this enzyme. The optimum pH values of flavin reduction by cellobiose (pH 4.5–5.0) and of electron transfer from flavin to heme (pH 3.5–4.0) differ from each other, and the rate-limiting ... Thiswas expected from the thermodynamic result that the redox potential of heme in F166Y was lower than that of WT. However, when the electron transfer rate k(s)1) and the driving force DG...
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Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf
... these residues stabilize the distortion of the NAG3 residue. In these interac-tions, we particularly focused on the Trp664 residue,which is located at the bottom of the active site cleft. The ... Stereo figures of the model of the bound substrate in the AD2 E526A mutant (A) and D524A mutant (B). The structures of boundsugars and the side-chains of three acidic residues in the conserved ... change in the conformation of the Glu526side-chain. The 2Fobs) Fcalcelectron density of the Glu526 side-chain in the D524A–substrate complex isnot clear compared with that of the wild-type...
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Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
... determined pI of 6.1 and the theoretically deduced pI value of 6.22 wasfound.A theoretical prediction of the secondary structuralorganization of Ag-NPA-1 performed on the basis of the amino acid ... cellulardistribution of the protein. The binding affinities arecharacterized by changes in the fluorescence of the singleTrp chromophore that is used as a marker of the proteinconformation and of the thiol ... uterus (ut) the intestine (i) and the parts of the pseudocoel (ps) stained with the pre-immune serum as control. (B) Consecutivesection of A showing intense staining of the pseudocoel (ps)...
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Báo cáo khoa học: Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida pptx
... to that of PMT-C3 is that,at the sequence level, there is no discernible similarity of PMT-C3 to the proteins, with the exception of the Cryseobacterium protein glutaminase. However, thereis ... preferential cou-pling of the Gaqversus Ga11protein to the down-stream PLCb effector protein. The PMT-induced PLCb response is potentiated by the release of the Gaqsubunit from the heterotrimericGabc ... is the detection of the modification, whichinvolves the detection of a molecular mass change of 1 Da in a relatively large protein substrate (20–45 kDa).Another challenge is the development of...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... influence the efficacy of interference RNA in vivo, for example, the length of target mRNA, the length and concentration of dsRNA, the region of homology between the dsRNA and the target, as well as other ... identity with the CHH from other penaeid species. In addition to anidentical number of amino acid residues (72), 13 of these were completely positionally conserved with allother members of the CHH ... unrelateddsRNA. They suggest that the complete silencing of the gene transcript could not be achieved due to dilu-tion of dsRNA in the feeding stage of the female tick[31]. On the other hand, Acosta and...
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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
... the remaining four subunits (NdhH–K) form the connect-ing subcomplex, both of them homologous to the bac-terial subcomplexes. The theoretical molecular mass of the Ndh complex, calculated from ... However, there is not much informa-tion about the existence of the soluble subcomplex (which is the electroninput device in bacterial complex I) in the composition of the Ndh com-plex. Furthermore, ... transcription of the ndh genes is much higher in BS chloroplasts, andelevated amounts of the Ndh complex have been foundin these plastids [18]. The function of the Ndh complex is still a matter of debate....
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Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf
... stable modification of the protein. However, the elution profiles from the various columns demonstrate that anoxia exposure didnot stimulate the synthesis of any new isozymic forms of GST. Based on ... change in the expression pattern of eitherisozyme during anoxia or of the expression of novelGST isozymes under anoxia. However, the effect of anoxia exposure on liver GST activity was profoundwhen ... were run in the absence of either GSH orenzyme. One unit of activity is defined as the amount of enzyme that formed 1 lmol of product per min at 21 °C.Turtle liver GST purification The purification...
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Báo cáo khoa học: Solution structure and stability of the full-length excisionase from bacteriophage HK022 pot
... to otherproteins of this class, such as the DNA-binding domain of the Mu repressor [21]. Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36and 41–44 of ... functionsobtained under the same conditions as the NMR experi-ments (Fig. 2). Direct comparison of the thermodynamicparameters of Xis_wt and Xis_C28S indicated that the influence of the Cys28 fi Ser ... shown). The influence of pH on the Xis_wt thermal denaturaion isillustrated in Fig. 1B. A visible increase of the proteinstability was observed when the pH was raised from 4.7 to7.0. At pH 4.7, the...
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Báo cáo khoa học: "Structural and functional characterization of the 5’ upstream region of a glutamine synthetase gene from Scots pine" pdf
... into the plasmid pGEM-3Z to generate the clone pGS217 and used for analysis and sequencing.2.2. Fusions of the 5’region of GS1a to the GUSreporter gene The 981 bp sequence upstream of the translation ... the isolation and characterization of a genomic clone encoding Scots pine (P. sylvestris) cytosolic glutamine synthe-tase GS1a. The clone contains the 5’ half of the gene including part of the ... analyzed the presence of putative elementsin the 5’ region of the gene. There is a canonical TATA box at–35 bp from the transcription start site and a putative CAATbox at –138 bp. The 5’ region...
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