... tissue. Finally, CO in cigarette smoke may havedampened the inflammatory response, especially in theearly phase. After continuous smoking the damaging andirritating effects may prevail, giving ... a monoclonal antibody against CD68 (IgG1 isotype, Dako,Glostrup, Danmark) as a marker for macrophages andrabbit polyclonal antiserum against iNOS (TransductionLaboratories, Lexington, KY, USA).The ... theinflammatory response in exhaled air, induced sputum,blood and urine. We hypothesised that smoking of twocigarettes would induce an increase in inflammatory cellsand markers within a limited...