... intensity of the upper band for both GC-boxes 1 and 2 in both cell lines, suggesting that the binding of Sp1, detected by the upper band, was inhibited by the antibody. On the other hand, pre-incubation ... out using nuclearextracts obtained from SH-SY5Y and HEK293 cells.As shown in Fig. 5A, the binding of nuclear proteinsto GC-boxes 1 and 2 was detected by doublet bands in both cell lines. The ... of anti-Sp1 and anti-Sp3 IgGs. The upper band disappeared in the presence of an anti-Sp1 IgG, while the supershift of the lower band in the presence of an anti-Sp3IgG was depicted. The binding...