... [ibuprofen],respectively, and kþoffis the koffvalue obtained in the absence of ibuprofen.Data analysisKinetic and thermodynamic data were analyzed with the matlab program (The Math Works, Natick, MA, USA). The ... cleft FA1 and the ligand-binding pockets FA2 and FA6, all located in domains I and II of (t)HSA.AbbreviationsFA, fatty acid; HSA, human serum albumin; HSA–heme-Fe, human serum heme-albumin; HSA–heme-Fe(III), ... denitrosylation has been investigated. The present dataindicate, for the first time, that the allosteric modulation of tHSA–hemeand HSA–heme reactivity by ibuprofen depends mainly on drug binding to the...