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Báo cáo y học: "Differential expression of RANK, RANK-L, and osteoprotegerin by synovial fluid neutrophils from patients with rheumatoid arthritis and by healthy human blood neutrophils" doc

Báo cáo y học:

Báo cáo y học: "Differential expression of RANK, RANK-L, and osteoprotegerin by synovial fluid neutrophils from patients with rheumatoid arthritis and by healthy human blood neutrophils" doc

... 1 Expression of RANK-L, OPG, RANK, and TRAF6 mRNAs by synovial fluid (SF) neutrophils isolated from patients with rheumatoid arthritis (RA) and by normal blood cellsExpression of RANK-L, OPG, RANK, ... value of less than 0.05.Results Expression of RANK-L, OPG, RANK, and TRAF6 mRNAs by SF neutrophils from patients with RA and by healthy human blood cellsFreshly isolated neutrophils from SF of ... Rochester, NY, USA).CytofluorometryThe expression of RANK-L and RANK at the membrane wasevaluated by cytofluorometry. Freshly separated healthy human blood or SF neutrophils from patients with RA and healthy...
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Báo cáo y học:

Báo cáo y học: "Pyridoxine supplementation corrects vitamin B6 deficiency but does not improve inflammation in patients with rheumatoid arthritis" pps

... period.Laboratory analyses Blood hematology and chemistry analyses and urinalysis wereperformed at the Clinical Laboratory of NEMC, Boston, MA.CRP concentrations were determined by enzyme immu-noassay kit ... tryptophanmetabolites by patients with rheumatoid arthritis. Arthritis Rheum 1964, 7:201-210.8. Mckusick AB, Sherwin RW, Jones LG, Hsu JM: Urinary excretion of pyridoxine and 4-pyridoxic acid in rheumatoid arthritis. Arthritis ... presence of functional vitamin B6 inadequacy in rheumatoid arthritis patients: ∆tHcy after a methionine load test was significantlyhigher in patients with rheumatoid arthritis than healthy matched...
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Báo cáo y học:

Báo cáo y học: "Differential expression of the angiogenic Tie receptor family in arthritic and normal synovial tissue" doc

... immunohistochemistry was performed with a rabbitantihuman Tie2 polyclonal antibody from Santa Cruz Biotechnology (Santa Cruz, CA, USA). (a) Synovial tissue from a patient with rheumatoid arthritis showing ... immunohistochemistry was performed with a goat antihuman Ang-1polyclonal antibody from Santa Cruz Biotechnology (Santa Cruz, CA, USA). (a) Synovial tissue from a patient with rheumatoid arthritis showingpositive ... 274:73-78.Supplementary materialMaterials and methodsPatient population Synovial tissues from patients with RA and OA, undergo-ing arthroplasty or synovectomy, who fulfilled the criteriaset by the American...
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Báo cáo y học:

Báo cáo y học: "Differential expression of chemokine receptors on peripheral blood B cells from patients with rheumatoid arthritis and systemic lupus erythematosus" ppsx

... differentiallyregulated.Methods Patients and controlsHeparinized whole blood (9 ml) from patients with RA, patients with SLE and patients with OA was obtained from the Depart-ments of Rheumatology and ... patients with OA, the expression of CXCR5 on B cells varied enormously in patients with RA and patients with SLE (Fig. 3). For patients with RA these valuesranged from 2.5 to 97.6% and for patients ... concentrations in sera. Sera of healthy controls and of patients with rheumatoid arthritis, systemic lupus erythematosus and osteoarthritis were tested in a standard ELISA test. A D450 of 0.06 cor-responds...
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Báo cáo y học:

Báo cáo y học: "Differential expression of the FAK family kinases in rheumatoid arthritis and osteoarthritis synovial tissues" doc

... acti-vated by a variety of extracellular stimuli [1]. FAK and Pyk2associate with the cytoskeleton and with integrin-signalingcomplexes by binding to Src kinase and paxillin [2-5]. FAK israpidly tyrosine ... number of cells of agiven type that reacted with a specific antibody divided by thetotal number of cells of that given type was defined as the per-centage of reactivity. The mean percentage of ... however, pPyk2 was highlyexpressed on synovial lining (pFAK = 15%, pPyk2 = 60%)compared to pFAK. The relative pattern of pFAK and pPyk2 expression was similar in OA patients& apos; synovial lining...
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Báo cáo sinh học:

Báo cáo sinh học: " Differential expression of papillomavirus L1 proteins encoded by authentic and codon modified L1 genes in methylcellulose-treated mouse keratinocytes" ppt

... morphology and expression of involucrin in the primary mouse KCs grown in KC-SF medium containing different concen-tration of methylcellulose for 2 daysFigure 1Cell morphology and expression of involucrin ... KCsEffects of post-transfection treatment of methylcellulose on expression of PV L1 genes in mouse primary KCsFigure 2Effects of post-transfection treatment of methylcellulose on expression of PV ... markers by reducing expression of basal keratins K14 and increasing expression of keratins K1 and K10 (datanot shown). These data indicate that methylcellulosecould induce mouse primary KCs to...
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Báo cáo hóa học:

Báo cáo hóa học: " Differential expression of papillomavirus L1 proteins encoded by authentic and codon modified L1 genes in methylcellulose-treated mouse keratinocytes" pptx

... morphology and expression of involucrin in the primary mouse KCs grown in KC-SF medium containing different concen-tration of methylcellulose for 2 daysFigure 1Cell morphology and expression of involucrin ... were analyzed by quantitative RT-PCRfor the L1 and tubulin transcripts using SYBR® Greet PCRkit (Qiagen, Australia). The specificity of RT-PCR wasdetermined by the Rotor Gene Software and agarose ... KCsEffects of post-transfection treatment of methylcellulose on expression of PV L1 genes in mouse primary KCsFigure 2Effects of post-transfection treatment of methylcellulose on expression of PV...
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Báo cáo y học:

Báo cáo y học: "Stable expression of a recombinant human antinucleosome antibody to investigate relationships between antibody sequence, binding properties, and pathogenicity" doc

... I, Koffler D: Avidity of anti-DNA antibodiesin serum and IgG glomerular eluates from patients with sys-temic lupus erythematosus. Association of high avidity antina-tive DNA antibody with ... Significance of enzyme linked immunosorbentassay (ELISA) for antibodies to double stranded and singlestranded DNA in patients with lupus nephritis: correlation with severity of renal histology. Ann ... described above. Human IgG was purified from thesupernatant using a Protein A column and the product wasanalysed for purity by SDS–PAGE and quantified by spectro-photometry. Purified human IgG was...
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Báo cáo y học:

Báo cáo y học: "The value of animal models in predicting genetic susceptibility to complex diseases such as rheumatoid arthritis" ppsx

... hundreds of thousands, or even millions, of tests. It is thereforeestimated that materials from tens of thousands of patients and control individuals are needed to identify the majority of genetic ... and strengthen the power of in silico methodsfor QTL analysis [86]. Unfortunately, the use of many of these resources is limited by the strict MHC dependency of most arthritis models.Another ... understanding of thegenes and pathways that affect human diseases. They mayalso permit improved understanding of the animal models thatwe are so dependent on in the development of safe and efficient...
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Báo cáo y học:

Báo cáo y học: "Differential expression, function and response to inflammatory stimuli of 11β-hydroxysteroid dehydrogenase type 1 in human fibroblasts: a mechanism for tissue-specific regulation of inflammation" pps

... al.:Fibroblast-like synoviocytes derived from patients with rheu-matoid arthritis show the imprint of synovial tissue heteroge-neity: evidence of a link between an increased myofibroblast-like phenotype and ... Sun K, Myatt L: Enhancement of glucocorticoid-induced11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibrob-lasts. Endocrinology 2003, ... D, MeansTK, Chakraverty R: Expression of 11beta-hydroxysteroid dehy-drogenase type 1 permits regulation of glucocorticoid bioa-vailability by human dendritic cells. Blood 2005,106:2042-2049.39....
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