... to increasethe binding affinity by providing an additional contact withthe SH2 domain of SOCS-3 involving R94. A conforma-tional change induced by the phosphorylation of tyrosine Y4 31 may also ... Masuhara, M., Mitsui, K., Yokouchi, M.,Ohtsubo, M., Misawa, H., Miyajima, A. & Yoshimura, A. (1997)CIS, a cytokine inducible SH1 protein, is a target of the JAK-STAT5 pathway and modulates ... thioredoxin was taken as control for specific binding. Steady state binding values were taken for Scatchard-analysis for the determination of Kdvalues.Table 1. Calculated Kdvalues of the EpoR...