... both NAD and NADP givingDV values of 2.2 and 1.7,respectively, and DV ⁄ Km,phosphitevalues of 1.9 and 1.7, respectively. To bet-ter comprehend the relaxed cofactor specificity, the cofactor ... work, both the kin-etic mechanism and practical application of PTDH-E175A ⁄ A176R wereinvestigated for better understanding of the enzyme and to provide a basis for future optimization. Kinetic ... and benefits of regeneration of a number of cofactors [8,9,11,12].NAD(P)H is involved in approximately 80% of enzy-matic reductions accounting for over 300 known reac-tions, many of which have...