Báo cáo khoa học: "In vitro propagation of interspecific hybrids" pps

... afterwards as selectedclones. In vitro micropropagation isapplied because of the poor development of cuttings.Materials and MethodsEarly selection of trees (at age 4 yr) was ... In vitro propagation of interspecific hybrids in AlnusH. Sbay,J. Guillot,P. DanthuD. PratLaboratoire ... thebrowning of explants, and finally put into culturemedium. Some aspects of in vitro culture weretested to improve the techniques.ResultsBasal culture medium for in vitro...

Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf

... of DDI-1A was better than that of DDI-1B, presum-ably because of the different conformation of the agly-con moiety. DDI-1A has a right-handed ... combinations) of di- or trinucleotiderepeats [36]. The efﬁciency of reiterative synthesisdepended on several factors including the length of the repetitive unit, its sequence and the characteristics of ... DiscussionEffect of DDI-1A and DDI-1B on repeatsexpansionDDI-1A and DDI-1B were tested for their effect onthe expansion of several doublet and triplet repeats inthe presence of the Klenow fragment of DNA...

Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc

... ratio of DNA/protein of 1.09 : 1 and anaverage ratio of RNA/protein of 1.12 : 1.Multiple-turnover (substrate in excess) DNA cleavagereactions were also performed to estimate the stoichiometry of ... of the pre-RNA reacted with or without preboundDNA. We investigated the robustness of this experiment byperforming it independently a total of three times using twodifferent preparations of ... reaction conditions of the inhibition experiment(Fig. 6). The rate of the release of bound, labeled DNA wasthen followed after adding excess unlabeled, pCOBLEDNA (Fig. 6D). A koﬀ of 0.07 min)1was...

Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot

... Palmer, J.M. (1985) Solubilization of the alternativeoxidase of cuckoo-pint (Arum maculatum) mitochondria. Stimu-lation by high concentration of ions and eﬀects of speciﬁcinhibitors. Biochem. ... itsstrong stimulation of respiratory rates; Fig. 1). It is there-fore unlikely that the inability of PTOX to use ubiquinone isan artefact of this assay (which relies on the ability of themembranes ... structural and catalyticfeatures. However, a number of established characteristics of AOXs, such as regulatory mechanisms [12–15] and theuse of iron as a cofactor [16–19], have not been assessed forPTOX....

Báo cáo khoa học: "In vitro susceptibilities of Leptospira spp. and Borrelia burgdorferi isolates to amoxicillin, tilmicosin, and enrofloxacin" potx

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Báo cáo khoa học: "In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs" pdf

... effectiveness of chordoma radiotherapy[17].It is sometimes difficult to perform complete radicalresection of chordoma tumors, depending on anatomiclocation or grade of tumor spreading. Because of thelower ... LH:Phase II study of 9-nitro-camptothecin in patientswithadvanced chordoma or soft tissue sarcoma. JClinOncol 2005,23(15):3597-3604.15. Hof H, Welzel T, Debus J: Effectiveness of cetuximab/gefitinib ... 5 of 9kinds of beams having different LET values, using Luciteabso rbers with various thicknesses to change the energy of the beams. At the sample position, we estimated theLET val ues of...

báo cáo khoa học: " In vitro behaviour of endothelial cells on a titanium surface" doc

... highdegree of biocompatibility of titanium and its alloys isintimately related to the passively formed oxide film onthe metallic surface [9,10].Noteworthy, the evaluation of the interaction of cells ... addition to the analysis of maintenance of cell via-bility. Indeed, the interaction of implants with host cells,and in particular with endothelial cells, might cause acti-vation of adhesion molecules ... with cytokinegeneration [2]. In fact, the degree of expression of adhe-sion molecules on the surface of human ECs depends onthe response of the cells against the implanted material[11].PECAM-1...

báo cáo khoa học: " In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration" pptx

... substrate of variouscell types, permits the in vitro maintenance of cells over along period of time and stimulates cell proliferation [23].Alpar et al. [21] evaluated the cytocompatibility of resorb-able ... time periods in vitro. The rate of cell proliferationwith time was different among the membranes examined. Of the six barriers tested, TD and RD demonstrated thehighest rate of HGF proliferation ... betweenEffects of various bioabsorbable and nonresorbable membranes on proliferation of human osteoblast-like (HOB) cells after 1, 2.5, 4, 24 and 48 h of incubationFigure 3Effects of various bioabsorbable...

báo cáo khoa học: " In vitro proliferation of human osteogenic cells in presence of different commercial bone substitute materials combined with enamel matrix derivatives" pdf

... d.BioMed CentralPage 1 of 9(page number not for citation purposes)Head & Face MedicineOpen AccessResearchIn vitro proliferation of human osteogenic cells in presence of different commercial ... 1Department of Orthodontics, Rheinische Friedrich-Wilhelms-University, Bonn, Germany, 2Department of Oral and Maxillofacial Surgery, Johannes Gutenberg-University, Mainz, Germany, 3Department of ... and methods: The aim of this study was to evaluate the proliferative activity of humanosteogenic cells after incubation over a period of seven days with commercial BSM of various originand...

báo cáo khoa học: " In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers" ppt

... 7:15http://www.head-face-med.com/content/7/1/15Page 3 of 9RESEARC H Open AccessIn vitro analysis of the cytotoxicity and theantimicrobial effect of four endodontic sealersInes Willershausen, Angelika ... means of the Alamar Blue assay. Cell growth and morphology was visualizedby means of fluorescent dyes. Possible antibacterial properties of the different sealers were visualized by means of SEM ... Theinduction of a mild tissue reaction, together with cellu-lar resorption of the sealing material in the case of extrusion over t he apical foramen, needs to be evalu-ated. Several in vitro, in...

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