... (1998)Dual regulation of a heat shock promoter duringembryogenesis: stage-dependent role of heat shock ele-ments. Plant J 13, 437–446.27 Diaz-Martin J, Almoguera C, Prieto-Dapena P, Espin-osa ... 3-amino-1,2,4-triazole; BiFC, bimolecular fluorescence complementation; EGS, ethylglycol bis(succinimidylsuccinate); EMSA,electrophoretic mobility shift assay; HS, heat shock; HSE, heat shock element; Hsf, ... HS promoters to sense and respond to heat ismainly due to the presence of consensus sequencescalled heat shock elements (HSEs). The eukaryoticHSE consensus sequence has been defined by alteringunits...