Báo cáo khoa học: " Identification and antigenic site analysis of foot-and-mouth disease virus from pigs and cattle in Korea" doc

Báo cáo khoa học: "Variable number tandem repeat analysis of Mycobacterium bovis isolates from Gyeonggi-do, Korea" ppt

... because of two different genotypes of the M. bovis strains. Interestingly, the pattern of the out-breaks at one cattle farm indicated a combination of the re-lapse of a previous M. bovis strain and ... collection and bacteriology Fifty-nine M. bovis isolates originating from dairy cattle that had tuberculosis-like lesions, and the cattle were from Gyeonggi-do livestock, were included in this ... loci of the ge-nome [10,22]. In eukaryotes, tandem repeated sequences, such as the microsattellites of 1-15 bp and minisatellites of 10-100 bp, have been found and used clinically [20]. In mycobacteria,...

Báo cáo hóa học: " Bit Error Rate Performance Analysis of a Threshold-Based Generalized Selection Combining Scheme in Nakagami " docx

... Netherlands,September 1999.Ahmed Iyanda Sulyman was born in Nige-ria in 1968. He obtained a Bachelor of Engineering (B.Eng.) degree in electricalengineering from the University of Ilorin,Nigeria, ... full ranking). For L = 5, M-GSC requiresa precombining processing of 10 comparisons and 30data swaps, while T-GSC requires 8 comparisons and Table 1: Precombining processing of M-GSC and T-GSC.Diversity ... Engineer II till he joined King Fahd University of Petroleum and Minerals (KFUPM), Saudi Arabia, as a graduatestudent in September 1998. He obtained his M.S. degree in electri-cal engineering...

Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

... reporter vector containing a muta-tion of the first binding site showed no response toaltered miR-23a expression, suggesting that the firstbinding site in the 3¢ UTR of IL6R mRNA mainlyreacted with ... interacts with the first potential binding site of the IL6R mRNA 3¢ UTR. (A) The three EGFP reporter vectors bear-ing mutations of the miR-23a seed region binding site are shown. The arrows indicate ... containing mutations of the second or third miR-23a binding regions. (C) Similarly, overexpression of miR-23a failed to suppress the EGFP intensity in the reporter vector containing mutation of...

Tài liệu Báo cáo khoa học: Speciﬁc interaction between the classical swine fever virus NS5B protein and the viral genome pdf

... sequence of 3¢ terminal of the minus-strand genome is essential tobinding of the CSFV NS5B protein. The minus-strand3¢ UTR with deletion of the 21 nucleotide fragment at theterminal did not interact ... mechanism of initiation from the very 3 ¢ termina l of 3¢ UTR might be one of peculiarities of CSFV replicationbecause the sequence of the 3¢ terminal of 3¢ UTR isnecessary fo r binding of NS5B ... swine fever virus (CSFV) is the causative agent of swine fever, which is a h ighly contagious and f atal viral disease o f pigs. CSFV, bovine v iral diarrhea virus (BVDV), and Border Disease virus...

Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx

... NTBI and TBI have at least onecommon step in uptake by hepatocytes [25–27].Fig. 6. Kinetics of NTBI uptake and incorporation into ferritin in nor-mal and iron-loaded cells. (A) Kinetics of NTBI ... already incorporated into ferritin.DFP has been postulated to exert an effect on theintracellular labile Fe pool and ferritin in cancer cells(melanoma), by diffusing into cells and chelating Fe from these ... chelators including DFO and DFP, act in part intracellularly, mobilizing Fe from ferritin butforming Fe-chelator complexes too hydrophilic to permeatecell membranes, thus accumulating in the cytosol.Tren-N-Me-3,2-HOPO...

Báo cáo khoa học: Comparing the substrate speciﬁcities of cytochrome c biogenesis Systems I and II docx

... toindicate the presence of the speciﬁc holocytochromeunder investigation if the features of the spectrum, in terms of wavelength maxima, and the position and intensity of heme-staining bands ... subdivided into proteins whichcontribute to the handling of heme and ligation of heme to the apocytochrome, and those involved in the provision of reductantto the apocytochrome in order to ... disulﬁde bond in the CXXCHheme-binding site. CcmH, which in someorganisms is two separate proteins CcmH and CcmI [50], appears to be involved in both heme handling ⁄ ligation and reductantprovision...

Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artiﬁcially introduced cross-links in proteins and protein complexes potx

... Candidate cross-links found in NK1 using BS3as a cross-linking agent. The cross-link candidates are nominated by theVIRTUALMSLAB program by assigning peaks in the MALDI-TOF massspectrum of ... spectrometric analysis of digests of cross-linkedproteins is known to be a powerful way to identify sites of cross-linking [3,6,8]. However, the identiﬁcation of cross-linked sites in biological ... fragment of HGF ⁄ SF was expressed in theyeast Pichia pastoris and puriﬁed from culture supernatants[22].Cross-linkingProtein cross-linking was carried out with BS3by incuba-ting the protein...

Báo cáo khoa học: Frontal afﬁnity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for afﬁnity to agalactosylated N-glycans potx

... fucosyla-tion and (d) there is a signiﬁcant inhibitory effect of the addition of bisecting GlcNAc.Binding of DC-SIGN-related lectins toagalactosylated glycoproteins In order to investigate the binding of ... analysis of DC-SIGN-related lectin-Fc chimeras.Fig. S4. Binding of DC-SIGN-related lectin-Fc chime-ras to Lec1 cells.Fig. S5. Structural analysis of binding sites of DC-SIGN-related lectin ... performed inhibition tests using a GlcNAc-binding lectin from Psathyrella velutina (PVL). WhenPVL (1 mgÆmL)1) was pre-incubated with Lec8 cells,binding of DC-SIGN–, DC-SIGNR– and LSECtin–Fcwas...

Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc

... thecorrect arrangement of the FAD-binding fold. Variations in dimer formation and unique colour changes of mutantproteins argue in favour of an interaction between theN-terminal cysteine pair with the ... of the nine present in human Alrp and in addition, the mutatedyeast proteins can be easily checked for in vivo activity.Materials and methodsStrains and plasmidsThe following strains of S. ... modelling and the energy minimizing of theFig. 1. Comparison of Erv1p, DN-Erv1p and Erv2p. The ﬁgure displays the correspondingcysteine residues and identity values for theN- and the C-terminal...

Báo cáo khoa học: Curcumin suppresses the dynamic instability of microtubules, activates the mitotic checkpoint and induces apoptosis in MCF-7 cells ppt

... combination with 8 lm curcumin, these concentrations of vinblastine inhibited proliferation by 44% and 51%,respectively. The CI values for the combination of 8 lm curcumin with 2 and 3 nm of vinblastine ... streptomycin, amphotericin B and peni-cillin and 10 lgÆ mL)1 of human insulin at 37 °Cinahumidiﬁed atmosphere of 5% CO2[48]. Curcumin stocksolution was prepared in 100% dimethylsulfoxide and ... μMCurcumin 36 μMControlCurcumin0 min15 min15 min25 min25 min0 minABFig. 2. Curcumin-perturbed mitotic spindle structures of MCF-7 cells. (A) MCF-7 cells were incubated without or with 24 and...

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