... the ter-tiary structure of ApeSOD has not been elucidated.In the present study, for the first time, we describe the crystal structure of ApeSOD. In particular, we focuson the coordination of ... ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions. The manga-nese was only 0.06 A˚out of the equatorial plane(Table 3). The angles around the metal ... metal cofactor.Figure 3D shows the superimposition of the activesite structures of apo, Mn-bound and Fe-bound Ape-SODs. The most significant difference among themwas related to the OH of Tyr39,...