... Gln 15 and the carbonyl oxygen atoms of Asp11 and Asn23 (Fig. 3) in an arrangement similarto what is observed in VPRK. Both PRK and VPRKhave calcium bound at Ca3. SPRK also has an aspar-tic acid ... 1022–10 25. 39 Perrakis A, Harkiolaki M, Wilson KS & Lamzin VS(2001) ARP ⁄ wARP and molecular replacement. ActaCrystallogr Sect D Biol Crystallogr 57 , 14 45 1 450 .40 Jones TA, Zou JY, Cowan SW & Kjeldgaard ... subtilases is capable of accommodating at least sixamino acid residues (P4–P2¢; notation according toSchechter and Berger [14]) ofa polypeptide substrateor inhibitor [ 15] , and both main chain and...
... Significantly, this structural feature of NOXtp is highly similar to that of valosine-containingprotein-like ATPase from Th. acidophilum, an archaealmember of the AAA family (ATPases associated ... theutilization of oxygen by Desulfovibrio gigas. Eur J Bio-chem 216, 443–448.32 Sakuraba H, Takamatsu Y, Satomura T, KawakamiR & Ohshima T (2001) Purification, characterization,and application ofa ... primer, 5 -CCG CAG GTT CCG GCG ATA GAGGGC GCC CAC CTG GAA GGA GTA TTC ACA GCA-3¢; and C12 2A reverse primer, 5 -TGC TGT GAA TACTCC TTC CAG GTG GGC GCC CTC TAT CGC CGG AACCTG CGG-3¢. The PCR was...
... 242–2 45. 24 Maeda T, Takekawa M & Saito H (19 95) Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor. Science 269, 55 4 55 8. 25 Posas F, Wurgler-Murphy SM, Maeda ... cerevisiaeMAP kinase cascades typically composed of three tiers of protein kinases, a MAP kinase (MAPK), a MAPK kinase(MAPKK) and a MAPKK kinase (MAPKKK), arecommon signalling modules in eukaryotic ... andKholodenko et al. [19]. Among them, two recent remark-able developments are that of Kholodenko et al. [14]based on stationary experimental data, and that of Sontag et al. [ 15] based on time-series...
... HinCel 6A and CcCel6C (Fig. 2)indicated that Asp 150 and Asp334 of CcCel6C arethe potential catalytic residues and could act as a proton donor and a base, respectively. Another aspar-tic acid ... Igarashi3,Masahiro Samejima3, Kiyoharu Fukuda1, Atsushi Nishikawa2and Takashi Tonozuka21 Department of Environmental and Natural Resource Science, Tokyo University of Agriculture and ... et al. 153 6 FEBS Journal 277 (2010) 153 2– 154 2 ª 2010 The Authors Journal compilation ª 2010 FEBScrystal structureofa cellulase was reported; it was a catalytic domain of Hypocrea jecorina Cel 6A (HjeCel 6A, ...
... linksBernard J. Premraj1, Swaminathan Raja1, Neel S. Bhavesh2, Ke Shi3, Ramakrishna V. Hosur2,Muttaiya Sundaralingam3and Narayanarao Yathindra11Department of Crystallography and Biophysics, ... J., Den Hartog, J .A. J., Van Boom, J.H. & Altona, C.(1981) Conformational analysis of the nucleotides A2 ¢ -5 A, A2 ¢ -5 - A2 ¢ -5 AandA2¢ -5 U from nuclear magnetic resonance andcircular dichroism ... considered fordetailed discussion.Calculated values of X-displacement and slide for thebase pairs in AFI are given in Table 5 . Average values of X-displacement and slide of GC base p airs at the GG...
... organicrinorganichybrid materials due to their applications in catalysis,sorption, energy storage, molecular electronics, optical ma-wxterials and ceramics 1–4 . A promising synthetic routetakes advantage ofa ... novelwxporous and low-dimensional materials 5 7 . Typical ex-amples include coorperative assembly of organicwxaminesrtransition metal oxides involving iron 8,9 , cobaltwx wx w x10 , vanadium 11 and ... low-temperature soft approach, hy-drothermal method, and the structure- directing function of organoamines. Recent developments in this area haveproven its viability for the successful preparation of...
... statisticalquantity for assessing the accuracy of crystal structures.Nature 355 , 472–4 75. 49 Cruickshank DW (1999) Remarks about protein structure precision. Acta Crystallogr 55 , 58 3–601. 50 ... mature a bacterial class I c-type cytochrome, Paracoccus denitrif-icans cytochrome c 55 0[33]. Moreover, many taxa thathave heme lyase apparently have separate heme lyasesfor the maturation of ... glycerol-3-phosphate dehydrogenase of Trypanosomatidae and theglycosomal redox balance of insect stages of Trypanoso-ma brucei and Leishmania spp. Mol Biochem Parasitol149, 155 –169.16 van Hellemond...
... kinetic parameters were determined at various concen-trations of the substrate, tamarind seed xyloglucan (Dainip-pon Sumitomo Pharma, Osaka, Japan), in 50 mm sodiumacetate buffer (pH 5. 5) at 45 °C ... at both ends. In the case of Xgh7 4A, the active cleft is open. Although a precise anal-ysis of the mode of action of Xgh7 4A has not beenperformed, Xgh7 4A appears to be an endoglucanasebecause ... used as a search model. The cns program [19] was used forrefinement against the 20–2 .5 A ˚intensity data. A ran-domly selected portion of the diffraction data (5. 0%)was used to calculate the...
... respectively, bymeans of standard Lineweaver–Burk analysis of initial ratekinetics. Fitting of the experimental data required as inputdata the initial concentrations of BApNA, enzyme andinhibitor, ... LCI-1.7 and a Discussion of Atypical Binding Sites of Bow-man-Birk Inhibitors. J Agric Food Chem 52 , 4219–4226.23 Catalano M, Ragona L, Molinari H, Tava A & Zetta L(2003) Anticarcinogenic ... generation of the solution struc-ture. Statistics for the total amount of experimentaldata are reported in Table 3. A simulated annealing (SA) procedure was usedstarting from a randomly generated...
... (GenBank: AAA19802),T. reesei (GenBank CAA4 959 6), A. niger (GenBankAAF04491), H. grisea (GenBank AAD11942) and A. acule-atus (GenBank BAA 251 83) gave sequence identity values of 65% , 64%, 73%, 51 % ... outer andinner RACE primers were as follows: outer 5 -RACEprimer CATGCGGTAAGGGTTGAAGTCACA-3¢;inner 5 -RACE primer 5 -GTTTGCTTCCCAGACATCCATC-3¢; outer 3¢-RACE primer 5 -ATGCTGTGGTTGGATTCCGACTAC-3¢; ... GenBank AAA19802), and Tricho-derma reesei (T.reesei; GenBank CAA4 959 6). Residues in white against a black background are amino acids that are identical or have a conservedsubstitution in all...
... pH 7 .5 8 .5] .Data collectionX-ray d iffraction data were collected using an i n-houseRigaku rotating anode X-ray generator with a MARResearch MAR3 45 image plat e detector. The radiationwavelength ... 276,307±326.26. Navaza, J. (1994) AMORE: an automated package for molecularreplacemen t. Acta Crystallogr. A5 0 , 157 ±163.27. Brunger, A. (1993) X-PLOR, V ersion 3.1. A System for X-RayCrystallography and ... designated as a a, h ead±tail, and b±b.Thea a dimer h as a diad3and ischaracterized as helix-helix packing between the twomonomers, as shown in Fig. 2A. The head±tail dimer isformed by a crystallographic...