... attached to the culture dish. Furthermore,cells expressing the JSP1-G2A mutant remainedattached to the dish despite the fact that the mutantprotein was expressed at similar levels to those of the wild-type ... protein for MS analysisof the myristoylation site, we used 293T cells as the expression system, as the presence of the SV40 largeT antigen in these cells enhances their resistance toapoptosis.Apoptosis ... in the floatingcells (Fig. 5C). Taken together, these data indicatedthat JSP1-wt, but not the -G2A mutant, induceddetachment of cells and induction of apoptosis, furtherdemonstrating that the...