... available at the end of the article Kataoka et al. AMB Express 2011, 1:10 http://www .amb- express. com/content/1/1/10© 2011 Kataoka et al; licensee Springer. T his is an Open Access article distributed ... mMMgCl2,5mMMgSO4,and250mMsucrose(TSB-plus Kataoka et al. AMB Express 2011, 1:10 http://www .amb- express. com/content/1/1/10Page 2 of 11 ORIGINAL Open AccessDevelopment of butanol-tolerant Bacillus ... experiments. Kataoka et al. AMB Express 2011, 1:10 http://www .amb- express. com/content/1/1/10Page 7 of 11decreased at 0.75, 1.0, 1.25, 2.0%v/v butanol for P.putida, E. coli, B. subtilis, (Nielsen et al....