... of trifluoroethanol on the conformation of RNase A withparticular consideration of the pretransition region of globalunfolding. In correspondence to reports by other authors [16,30,31],CD spectra in the near-UV ... (1999) In uence of disulfide bonds on the induction of helical con-formationinproteins.J. Protein Chem. 18, 481–488.17. Price, N.C. & Johnson, C.M. (1990) Proteinases as probes of conformation of soluble ... detectableincrease in the content of secondary structure only after the disruption of the native tertiary structure of RNase A(Figs 2–4). Interestingly, the preservation of the tertiarystructure coincides...