... Simone R, Columba-Cabezas S, Penna G, Adorini L:Functional maturation of adult mouse resting microglia intoan APC is promoted by granulocyte-macrophage colony-Journal of Neuroinflammation ... need for additional microglia cul-tures. In addition, the main inflammatory responses of microglia following S. aureus stimulation, namely proin-flammatory mediator production and phagocytosis, ... overnight at 4°C. The following day, cellswere incubated with a donkey anti-rat biotinylated sec-ondary antibody (Vector Laboratories, Burlingame, CA)and detected using a streptavidin-Alexa Fluor...