... ResearchOpen AccessResearch article In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNAKorakot Nganvongpanit*1, Patama Chaochird2, ... important type of collagen in the ECM. This research also focuses on the effect of the suppression of the MMP-3 gene on mRNA and proteoglycan production. Moreover, the biologicaleffects of the suppression ... and stored in culture media at -20°C.Measurement of GAG levels The level of GAG appearing in the medium of explants,cell cultures, and papain-digested cells was determined using the dimethylmethylene...