... and the amount of reCBG in thesupernatant w as estim ated b y activity measurement ass aysusing 4NPGlc a s substrate. Expression of cbg-1 in P. pastoris and isolation of reCBGLarge-scale expression ... beta-glucosi-dase. Patent WO 99/03874.36. Yahata, K., Mori, K., Arai, H., Koide, S., Ogawa, Y., Mukoyama,M.,Sugawara ,A. ,Ozaki,S.,Tanaka,I.,Nabeshima,Y.&Nakao,K. (2000) Molecular c loning and e ... nucleotides encoding a p rotein of 496 a mino acids with a calculated molecular mass of 53.7 kDa. A singleputative glycosylation site was located at N47 of thededuced amino-acid sequence within the motif...