... (1), with an r2 of 0.951, is based on the elution data of all four proteinstandards. Linear regression (2), with an r2 of 0.981, is based on the elution data of the 200, 150, and 66 kDa protein standards. ... correlated with the growth temperature of the sourceorganism, with the notable exception of BLXI. It is alsointeresting that of the B. licheniformis proteins with sequence available, the Q and N ... BLXI activity in the presence of Mn2þ and Mg2þreached only 6% and 15%, respectively, of the activityin the presence of Co2þ(Fig. 4A). Co2þ,Mn2þ, and Mg2þactivation constants for BLXI...