... 5'-TTTCTCCTGGTATGAGATAGC-3', TNFR1 forwardprimer: 5'-CTAAACAGCAGAACCGAGTGT-3', TNFR1reverse primer: 5'-AGATACGTAGAGTGTCCTTGG-3',TNFR2 forward primer: 5'-ATAAAGCCACAC-CCACAACCT-3', ... quick-frozen brains with Isogen (Nippon gene, Toyama, Japan). The random 9-mers-primed cDNA was prepared with an RNA-LA-PCRKit (Takara Shuzo, Kyoto, Japan) and 2 µg of total RNA. A LightCycler PCR and ... J, Tsukamoto H, Yanagihara I, Ozono K, Okada S: Molec-ular cloning and expression of cDNA for murine galactocer-ebrosidase and mutation analysis of the twitcher mouse, a model of Krabbe's...