... arrestin, binds with high affinityto the phosphorylated receptor to sterically inhibit the interaction of the receptor with G protein, resulting in the shutting-down of the downstream signaling [1–4].Vertebrate ... FRYGRED and PCSVMLQ, respectively.Finally, based on the nucleotide sequence of the PCRproducts, specific primers were synthesized and used for5¢ -and3 ¢-RACE. Based on the sequence of 5¢ -and3 ¢-flanking ... system. Upon the exposure of 10 lMisoproterenol, b2-AR agonist, the numbers of the receptor at the cell surface with and withoutoverexpression of exogenous Ci-arr decreased by about 30 and 60%...