... hosts such asE. coli [22], Saccharomyces cerevisiae [23], and actinomycetes [24].For example, Corynebacterium glutamicum, orig-inally a L-glutamic acid-secreting microorganism,was subjected ... se-quences, it has become possible to reconstructgenome-scale biochemical reaction networks inmicroorganisms. Over the recent years, genome-scale metabolic reconstructions for E. coli K- 12MG1655 ... 1216–1220.[14] Chockalingam, K. ,Chen,Z.L., Katzenellenbogen, J.A., Zhao,H. M., Directed evolution of specific receptor-ligand pairsfor use in the creation of gene switches. Proc. Natl. Acad. Sci.USA...