... backbone bytrifluoroethanol molecules, the proton donator/acceptorproperties of the trifluoroethanol molecule for hydrogenbonds and the influence of trifluoroethanol on the dielectric constant of the ... hydro-chloride (GdnHCl), urea or elevated temperatures, theydestroy the tertiary structure of proteins which results in the loss of enzymatic activity. Regarding the secondary struc-ture of ... Germany,for performing NMR spectroscopy experiments. The support for JensKo¨ditz by the Land Sachsen-Anhalt and by the Max-Buchner-Forschungsstiftung, Frankfurt/Main, Germany, is gratefully...