... (Promega),followed by assay of DNase I and b-galactosidase activities,according to a previously described method [32].Preparation of nuclear extracts and EMSAThe nuclear extract and probe were prepared as reportedpreviously ... Kawai4, Tamiko Nakajima1, ChikakoMakita1, Masako Itoi1, Yutaka Tajima1, Koichiro Kishi1 and Toshihiro Yasuda21 Department of Legal Medicine and Medical Genetics, Gunma University, Japan2 ... a slow-migrating major band and several faster-migra-ting faint bands. Alternative splicing occurs in manygenes, and so DNA fragments were purified from themajor band and cloned into a sequencing...