... products were cloned into a pTOPOBlunt vector (Invitrogen) and sequenced (pTOPO ⁄ hhipro 1& 2, pTOPO ⁄ hhipro 3). The DNA fragments were sub-cloned into a pGL3-basic vector (Promega) between ... anautomatic DNA sequencer (DSQ-1000, Shimadzu, Kyoto,Japan) after cloning into pGEM-T Easy vector (Promega,Madison, WI). Sequence analysis was performed with gen-etyx software (Genetyx, Tokyo, ... gene in human neuroblastoma cells. The human KLK11 promoterregion was linked to a firefly luciferase reporter gene in a pGL3 basic vector, and transfected into KP-N-YN neuroblastoma with the Renillaluciferase...