Báo cáo khoa học: Directed evolution of a glutaryl acylase into an adipyl acylase potx

... a- aminoadipyl side chains varies greatly. As the glutaryl, adipyl and a- aminoadipyl side chains are all verysimilar, it can be envisaged that a glutaryl acylase is a goodstarting point for directed ... Theenzyme deacylated glutaryl- 7-ACA with a catalytic con-stant kcat of 8.1 s)1and with a Michaelis constant Km of 0.08 mM. Adipyl- 7-ADCA is deacylated at a lower kcat of 0.65 s)1and a higher ... et al. (Eur. J. Biochem. 269) Ó FEBS 2002 Directed evolution of a glutaryl acylase into an adipyl acylase Charles F. Sio1, Anja M. Riemens2, Jan-Metske van der Laan2, Raymond M.D. Verhaert1,*...

Báo cáo khoa học: Directed evolution of a histone acetyltransferase – enhancing thermostability, whilst maintaining catalytic activity and substrate speciﬁcity doc

... his-tone acetyltransferase domain of the human PCAFtranscriptional regulator bound to coenzyme A. EMBOJ 18, 3521–3532.35 Ahmad S, Gromiha MM, Fawareh H & Sarai A (2004)ASAView: solvent accessibility ... substrate speciﬁc-ity. P ⁄ CAF, p300 ⁄ CBP-associating factor, is a transcriptional coactivator with a variable N-terminal, a central HAT domain and a C-terminal bromodomain.Several stabilizing ... evolution of a histone acetyltransferase –enhancing thermostability, whilst maintaining catalyticactivity and substrate speciﬁcityHans Leemhuis1, Karl P. Nightingale1,2and Florian Hollfelder11...

Tài liệu Báo cáo khoa học: Directed evolution of trimethoprim resistance inEscherichia coli doc

... compounds[12,13], and numerous studies of DHFR from manyorganisms [11]. The breadth of information availableon DHFR and the signiﬁcance of it in the treatment of a range of conditions makes it an ideal subject ... selection of drug targets. Along with a clear indication of the easewith which antibiotic resistance can develop and themeans by which it can do so, directed evolution canalso provide a pool of resistant ... Escherichia coliMorgan Watson, Jian-Wei Liu and David OllisResearch School of Chemistry, Australian National University, Canberra, AustraliaNaturally occurring enzyme inhibitors are found...

Báo cáo khoa học: Directed evolution of formate dehydrogenase from Candida boidinii for improved stability during entrapment in polyacrylamide ppt

... 4.1aaa fi agaVariants of second generationE53V ⁄ K56R ⁄ C23S gaa fi gta 120% a 1600 42aaa fi agatgt fi agt a Residual activity was measured in 15% AA ⁄ 0.1% TEMED.Stabilisation of FDH for entrapment ... step of evolution. Stability of mutant FDHs in PAAFinally, the performance of the FDH variants afterentrapment in PAA was investigated by measuring theactivity in comparison to equally entrapped ... PAA beads and3 mL hexane were placed into an 8 mL GC-vial, whichthereafter was sealed with a rubber cap and left to equili-brate to a temperature of 30 °C for 30 min. The reactionwas started...

Báo cáo khoa học: Crystal structure of a cold-adapted class C b-lactamase potx

... hydrolysis of the critical b-lactam ring and renderthe antibiotic inactive against its original cellulartarget, the cell wall transpeptidase. b-Lactamases of Keywordsclass C b-lactamase; cold adaptation;psychrophile; ... Lie`ge-Sart, Tilman, Belgiumb-Lactamases are the major causes of bacterial resis-tance to the b-lactam family of antibiotics, such aspenicillins and cephalosporins. These enzymes catalyzethe ... 2BLT) as a search model. The model wassolved to a resolution of 2.2 A ˚. A summary of datacollection and reﬁnement statistics is presented inTable 4. Ramachandran plots indicate that 87.7% of nonglycine...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... mutationsMousumi Banerjee1, Hemalatha Balaram2and Padmanabhan Balaram11 Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India2 Molecular Biology and Genetics Unit, Jawaharlal ... inenzymes: a study of triosephosphate isomerase andcomparison with methyl glyoxalsynthase. Adv ProteinChem 66, 315–372.45 Gunasekaran K, Ramakrishnan C & Balaram P (1996)Disallowed Ramachandran ... Structure and function of a regulated archaealtriosephosphate isomerase adapted to high temperature.J Mol Biol 342, 861–875.12 Gayathri P, Banerjee M, Vijayalakshmi A, Azeez S,Balaram H, Balaram...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... byinvertebrates, and has three crystal phases: calcite, ara-gonite and vaterite. Although calcite is the most stablecrystal thermodynamically, many organisms can formmetastable aragonite crystals ... region wasdecreased and a new band was detected at 64 kDa, butonly after treatment with heparitinase II (Fig. 5A) .Although the same band was obtained after digestionFig. 3.45Ca overlay analysis ... structure–functionrelationship analysis of Prismalin–14 from the prismaticlayer of the Japanese pearl oyster, Pinctada fucata.FEBS J 274, 5158–5166.9 Murayama E, Takagi Y, Ohira T, Davis JG, GreeneMI & Nagasawa H...

Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx

... absorption bands. Heme catabolism byHOs of mammals, pathogenic bacteria, cyanobacteriaand probably insects is considered to have a similarmechanism, because the characteristic absorptionbands of verdoheme ... higherplants have been identiﬁed in a few moss plants,several angiosperms (tobacco, tomato, pea, soybean,rice plant, sorghum, Arabidopsis thaliana), and a gym-nosperm (loblolly pine) [8]. So far, ... Then, a broad bandappeared at around 660 nm, and was maximal 9–12 minafter initiation of the reaction. The spectral features of the ﬁnal reaction mixture were analogous, but not iden-tical,...

Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of speciﬁcity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf

... smaller compound PEA(Fig. 1B) and a water molecule are present and PEXand PEA reﬁned satisfactorily with occupancies of 0.7Table 1. Data and reﬁnement statistics. Values in parentheses pertain ... 25% of the accessiblesurface area of a monomer contributes to LlPDHdimer formation. Whereas LlPDH and OaPDH have a buried surface area of  5500 A ˚2, TbPDH has a largerinterface surface area ... averageisotropic thermal parameters (B-factors) and, whereappropriate, the ligand occupancies.Superposition of subunit A on B and C (468 Caatoms) in complex II and III of LlPDH gives an rmsd...

Tài liệu Báo cáo khóa học: New activities of a catalytic antibody with a peroxidase activity ppt

... change of the Fe(II) spin state and noreplacement of any of the two axial ligands of the iron,His18 or RNO, by an amino acid side-chain of theantibody protein.Binding site topology of antibody ... theS-oxidation of thioanisole associated H2O2as an oxidantwith MP8 as a catalyst in the presence of tBuOH as an organic co-solvent (Table 2), the stereoselective S-oxidation of thioanisole ... spectrometry.Preparation of monoclonal antibodiesMP8 was covalently attached to keyhole limpet hemo-cyanin (KLH) and to BSA, using glutaraldehyde as a coupling agent, in 1Mbicarbonate buffer, pH 9.5, accordingto...

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