Báo cáo khoa học: Activated transglutaminase from Streptomyces mobaraensis is processed by a tripeptidyl aminopeptidase in the ﬁnal step pptx

... with the tetrapeptide Ala-Ala-Val-Ala-pNA was revealed. The inability of the peptidase to hydrolyse Ala-Ala-pNA andAla-pNA (or other chromogenic amino acids) at reason-able rates clearly indicates ... Activated transglutaminase from Streptomyces mobaraensis is processed by a tripeptidyl aminopeptidase in the ﬁnal step Jens Zotzel*, Ralf Pasternack*, Christiane Pelzer*, Dagmar Ziegert, Martina ... metalloprotease; TAP, tripeptidyl aminopeptidase; TGase, transglutaminase. Enzymes: transglutaminase, protein-glutamine:amine c-glutamyl-transferase from Streptomyces mobaraensis (EC 2.3.2.13; SwissProtentry...

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... bindingand degradation [63]. In particular, an interactionbetween the cytoplasmic domains of CD4 and VpU is no longer detectable in a yeast two-hybrid assay ifD77 of VpU is replaced by asparagine ... interaction. The distinguished protein confor-mation is then recognized by the binding partner.Directed withdrawal of a particular conformationalsubpopulation from the equilibrium is counteracted by ... distance con-straints [78], and dyana for calculation of preliminaryprotein structures [79]. radar runs in an iterative manner. The input of the ﬁrst cycle consisted of the 3D NOESYdata, the...

Báo cáo khoa học: Complementation of coenzyme Q-deﬁcient yeast by coenzyme Q analogues requires the isoprenoid side chain ppt

... h, at which point it had reached A 600 0.8 and already been sta-tionary for several hours and would remain that way for a number of days (squares). At the point indicated by the arrow, a mixture ... this was a valid assumption as loss ofarea from the CoQ2H2and decylQH2peaks was compara-ble with the gain in size of the CoQ2and decylQ peaks.Determination of the fraction of CoQ2and ... putative MATa Dcoq2::kanMX colonies were crossedwith the MATa Dcoq3::kanMX strain.As the MATa knockout library to which it would bemated carries a kanMX resistance marker, the strain had itskanMX...

Báo cáo khoa học: Autocatalytic processing of procathepsin B is triggered by proenzyme activity doc

... 5¢-CCAGAACGTTATGTTTGCAGCTGCACTGAAGCTGCCTGC-3¢(for the TED58AAA mutant: R54N, T5 8A, E5 9A, D6 0A) ;5¢-CCCAGAACGTTATGGCTGCAGCTGCACTGAAGCTGCCTG-3¢ (for the FTED57AAAA mutant: R54N,F5 7A, T5 8A, E5 9A, D6 0A) ; 5¢-CCAGAACGTTATGGCTACCGAGGACCTGAAGC-3¢ ... [3–5],rheumatoid arthritis and osteoarthritis [3,6].Cysteine cathepsins, including cathepsin B, are syn-thesized as inactive proenzymes, which are activated by other proteases or by autocatalytic ... 921kDaCoomassiestainingAutoradiographyFig. 3. Labelling of procathepsin B by 125I-DCG-O4. Five micro-grams of recombinant protein (pCatB, procathepsin B; CatB,cathepsin B; pCatB C29S, catalytic procathepsin...

Báo cáo khoa học: Stage speciﬁc expression of poly(malic acid)-afﬁliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx

... generated from ﬁrst-strand cDNAand the following primers (NKA48, accession numberDQ017261): 5¢-GATGCATAATACGACTCACTATAGGGAAATGTCCGTCCAACAAGGAG-3¢ (forward) and 5¢-GCCTTCTAATACGACTCACTATAGGGACCACGATGATGGATGAAATG-3¢ ... 5¢-GATGCATAATACGACTCACTATAGGGAGTGCCTTGCAAGGAGTATTG-3¢ and the reverse primer was 5¢-GCCTTCTAATACGACTCACTATAGGGAGCTCGTAATAGCTTTTGGAC-3¢, the resulting DNA spanning nucleotides21–536 of the gene (accession ... 1049lin 3a is the most abundant mRNA. During differenti-ation, synthesis of PMLA discontinues, and the remaining polymer is exported into the extracellularﬂuid and degraded. It is assumed that the...

Báo cáo khoa học: Speciﬁc degradation of H. pylori urease by a catalytic antibody light chain pptx

... natural catalytic antibodies have been discov-ered in the last decade. The ﬁrst natural catalytic anti-body was isolated from the serum of an asthmapatient [1], and this antibody enzymatically ... a Gram-negative spiral bacteriainfecting about 50% of the world’s population, is anetiologic agent in a variety of gastroduodenal diseasesand is the only microorganism known to inhabit the ... the catalytic light chain may contribute to the long lagphase [19].Using the intact H. pylori urease, a cleavage test wasalso performed. The cleavage sites conﬁrmed by N-ter-minal amino-acid...

Báo cáo khoa học: Regulation of luteinizing hormone receptor mRNA expression by mevalonate kinase – role of the catalytic center in mRNA recognition potx

... produceany change in the RNA binding activity of MVK(Fig. 5). As a decrease in the RNA binding activity ofMVK was observed when the amino acids at the activesite were mutated, we examined the ... hypothesis.Two structural scenarios that explain the present muta-genesis data are as follows: (a) the RNA binding siteoverlaps with the site of ATP binding and may exploitcommon side chain interactions; ... LHRmRNA binding and for its role as a translational sup-pressor of LHR mRNA. The results showed a sub-stantial decrease in LHR mRNA binding activitywhen any of the amino acids at the active...

Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx

... released from the glycoprotein fraction by the action of PNGase. They were then analyzed by HPLC after mild acid treatment and the sequential action ofb-galactosidase and b-hexosaminidase as ... Glc1Man5Glc-NAc2/Glc1Man9GlcNAc2 obtained with the glycoproteinpattern. This indicates that, when glycoproteins bearingMan9 and Man5 are synthesized in the same cell line, thosebearing Man5 are more ... processing,most of the Man5GlcNAc2 was converted into Man4Glc-NAc2, in contrast with the small amount of Man6GlcNAc2originating from the Man9GlcNAc2 species.ERAD of glycoproteins bearing Man5 and...

Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot

... e.g., after viral infection. Weshow that human IFN-b mRNA follows an asynchronousdeadenylation pathway typical of a mRNA containing a class II ARE. A deletion analysis of the IFN-b naturaltranscript ... c-fos, it was shown thatARE mediates mRNA deadenylation by a translation-independent mechanism, while the coding region instabi-lity determinant facilitates mRNA deadenylation by a mechanism coupled ... shown). These observationsindicate that deadenylation is a general mechanism con-trolling the length of IFN-b mRNA poly (A) tail.IFN-b mRNA deadenylation is mediated by both the ARE and the CRIDThe...

Báo cáo khoa học: Crystal structures of HIV protease V82A and L90M mutants reveal changes in the indinavir-binding site potx

... indinavir, of 3.8 A ˚ .In PRV8 2A , the change in the position of the main chainatoms placed the Cb atom of Ala82 within reasonable vander Waals distance of indinavir (4.1 A ˚), resulting in a ... residue in the active site of the protease that is critical to inhibitor binding, L90M is distal to the inhibitor-binding site and is located near the dimerization interface. The kinetic parameters ... hand, wasdisordered in all the structures and had poorer comple-mentarity for the van der Waals interaction with the indanylgroup. Analysis of the anisotropic displacement parameters,usingPARVATI[29],...

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