Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starﬁsh sperm and its induction by egg jelly triggering the acrosome reaction pdf

... 2003 In vivo cross-linking of histones (Eur. J. Biochem. 270) 3759 In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starfish sperm and its induction by egg jelly triggering ... is the firstdemonstration of an in vivo activation of transglutaminase leading to the formation of a cross-link in intracellularproteins.Keywords: starfish; sperm; transglutaminase; chromatin; acrosome ... isproduced by a nuclear transglutaminase which is able toutilize nucleosomal histone H2B as an amine acceptor. In the starfish, the jelly coat of the eggs cause sperm toundergo the acrosome reaction...

Báo cáo khoa học: Dissecting the role of the N-terminal metal-binding domains in activating the yeast copper ATPase in vivo pptx

... shown thattheir N-terminus modulates their activity [36,37]. The current hypothesis involves domain–domain interac-tions between the N-terminus and other domains of the ATPase. Indeed, in vitro ... studies of the puriﬁedN-terminus and catalytic loop of the Wilson ATPasehave shown that these two domains interact in the absence of copper and that their interaction is dimin-ished by copper binding ... N-terminus of the ATPase.To further study the role of the Ccc2 N-terminus in the overall function of the ATPase in vivo, we engi-neered DMBDCcc2 (Fig. 1E), a protein in which the N-terminus...

Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

... oxygen of the main chain at Asp266 was bound to amino groups of main chain of Tyr269 and Ala270 by hydrogen bonds. The interactions should maintain the structure of the C-terminalregion. In the ... region on the opposite side of the N-terminal region on the molecular surface. In the wild-type enzyme, the side chain of Asp266 interacted with the amino group of the main chain at Glu268. The oxygen ... than that of wild-type ALP I. On the otherhand, the Tm of D266N/Y269A-ALP I increased by only4 °C. The denaturation temperature indicates the stability of the structure of the protein. The difference...

Báo cáo khoa học: "LANGUAGE SYNTHESIS GENERATION OF GERMAN FROM CONCEPTUAL STRUCTURE: MT PROJECT IN A JAPANESE/GERMAN" pot

... REFERENCING AND FOCUSSING For referencing and other phenomena like focussing, the simple approach of only allowing a schema instance as a filler is not sufficient. We therefore included in our ... semantic synthesis. The project is funded by the "Informationslinguistik" program of the Ministry for Research and Technology (BM~T), FRG, and is carried out in cooc~ration with ~JJITSU ... feasibility of the approach of using a semantic representation as an interlingua in a practical application will be investigated and demonstrated by translating titles of Japanese papers from the...

Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc

... of the spermatocytes signal (anti-Stk33 in red and anti-a-tubulin in green). (D–I) Immunostaining combined with nuclear staining in mouse lung preparations (anti-Stk33 in red and nuclear staining ... 68% of the entries, and interestingly thereare no entries from the lung. In addition to testis, thereare single ESTs from retina, pituitary gland and cir-cumventricular organs of the brain ... signal was perinuclear, strongly supporting its association to germinal cells. No signal was observed in periluminar areas of the germinal epithelium or in spermatozoa, in cells from the interstitial...

Báo cáo khoa học: Proteomic identiﬁcation of all plastid-speciﬁc ribosomal proteins in higher plant chloroplast 30S ribosomal subunit PSRP-2 (U1A-type domains), PSRP-3a/b (ycf65 homologue) and PSRP-4 (Thx homologue) doc

... PSRP-2 contains twoRNA-binding domains which occur in mRNA processing/stabilizing proteins (e.g. U1A snRNP, poly(A)-bindingproteins), suggesting a possible role for it in the recruiting of stored ... group of RNA-binding proteins (cpRNPs) present in the chloroplast stroma. Other high-alignment hits were:polyadenylate-binding proteins, glycine-rich RNA-bindingproteins, heterogeneous nuclear ... wereobtained by sequencing P2-3/P2-4 and P4-3/P4-4 usingsequencing primers, TAG1 and TAG2, and the two strandswere completely sequenced by primer walking. The phageclone of PSRP-3 cDNA was obtained...

Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf

... petB, and to domain IV of the petB intron. The binding of the homodimer of HCF152 in this region somehow stabilizes the splicingproducts, possibly by folding the RNA into the correctsplicing ... located at the C-terminus of the protein although binding of the C-terminus half (CH) isonly half of the full-length protein (Fig. 3). Interestingly, the binding efficiency of the full-length mutant ... in the UV-crosslinkingcompetitive assays, as shown in D. The IC50 of each RNA wasdetermined by plotting the data (D). The location of the high affinitybinding site in the Dd120 RNA defined in...

Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf

... replaced by otherresidues in the authentic ribose-binding proteins. In addition, the repressor sequences include a DNA-bind-ing headpiece, and so are consistently longer thanthose of the binding ... protein. This kind of protein is exempliﬁed by the Thermus thermophilus protein, PDB entry 2B3B [28]. The mode of binding the monosaccharide is completelydifferent in terms of orientation of the ... following the ﬁrst helices of domains 1 and 2, respectively. The EF-hand-like calcium site of domain 2 was describedearlier, and tight binding of the ion was shown to con-tribute to the integrity of...

Báo cáo khoa học: The speciﬁcity of alcohol dehydrogenase with cis-retinoids Activity with 11-cis-retinol and localization in retina docx

... ADH4 is detected in the retinal pigment epithelium (RPE) and itis extensively distributed in the retina. ADH4is found in the outer (ONL) and the inner(INL) nuclear layers, in the inner plexiformlayer ... distributed in the inner layers of the retina (Fig. 3). ADH4 was present in the outer nuclear, inner nuclear, inner plexiform and ganglioncell layers. The signal was absent in the choroid and outerplexiform ... suggestingthat the distinct location of the substrate in the bindingpocket of ADH4 is the reason for the low activity observedwith this retinal isomer. The interaction of 11-cis-retinal with ADH1B1...

Báo cáo khoa học: The catalytic role of the distal site asparagine-histidine couple in catalase-peroxidases potx

... complex and the ferric protein) and the corres-ponding fit are shown in Fig. 4B. The observed rate of cyanide binding to the ferric enzyme linearly increased with the concentration of cyanide. The ... (i.e. Reinheitszahl) of Asn153fiAla varies between 0.46 and 0.48 from onepreparation to another (wild-type protein: 0.57–0.61). The Reinheitszahl of Asn153fiAsp is in the range 0.42–0.45 and the peaks ... oxidation rates of guaiacol and o-dianisidine as the wild-type protein(Table 1).Cyanide bindingCyanide is a useful probe to investigate the binding site of heme proteins. Figure 4A shows the spectral...

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