Báo cáo khoa học: Insights into the design of a hybrid system between Anabaena ferredoxin-NADP+ reductase and bovine adrenodoxin pot
Báo cáo khoa học: Insights into the design of a hybrid system between Anabaena ferredoxin-NADP+ reductase and bovine adrenodoxin pot
... heterologous system that consists of cyanobacterial FNR and adrenal bovine Adx. Anabaena PCC 7119 FNR contains a noncovalently bound FADgroup and its main physiological function is the transfer of two ... shown that ferredoxin-NADP+ reductase from Anabaena and adrenodoxin from bovine adrenal glands are able to form optimal complexesfor thermodynamically favoured electron transfer reactions.Thus, ... interaction (Eur. J. Biochem. 270) 735 Insights into the design of a hybrid system between Anabaena ferredoxin-NADP+ reductase and bovine adrenodoxin Merche Faro1, Burkhard Schiffler2, Achim...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... illustrates the quality of the experimental data(Fig. 2B).Reconstruction of the overall shape of the PLDa2from the X-ray scattering data was achieved by the ab initio modeling program dammin ... plot of the data from (A) in the range 0–100 mM Ca2+. (C) Modified Scatchardplot of the data from (B). DA corresponds to the change in relative activity. The PLDa2 activity was measured against ... spectrum of PLDa2 exhibits: (a) a sharp maximumat 192 nm; and (b) a wide minimum between 208 and 220 nm. The a- helix and b-strand contents of the protein were calculated with the online programsdichroweb...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... fully active state. In contrast, the arginaseactivity of the Asn149Asp variant was practicallyundetectable, both before and after the incubation with the manganese ions.Fully active His120Asn and ... 7.5, and then assayed for arginase activity in the absence of added Mn2+(open bars) and after full activation with the metal ion and assay in the presence of added 2 mM Mn2+(filled bars). Arginase ... considered for arginase and agmatinase[20]. Such substrate discrimination is particularlyimportant for mammalian arginase II and agmatinase,as both enzymes are mitochondrial and functionallydifferent...
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Báo cáo khoa học: Insights into the activation of brain serine racemase by the multi-PDZ domain glutamate receptor interacting protein, divalent cations and ATP ppt
... coli and the purification of two larger fragments of GRIP and incubated the purified proteins with SR to analysechanges in activity. A hexa-His tag was introduced at the N-terminal end and a FLAG ... and mediation of neuronal migration. Proc NatlAcad Sci USA 102, 2105–2110.13 Fujii K, Maeda K, Hikida T, Mustafa AK, Balkissoon R,Xia J, Yamada T, Ozeki Y, Kawahara R, Okawa M et al.(2006) ... other hand, PDZ7 is close to the carboxy-terminal end of the amino acid sequence[15]. The two clusters of PDZ domains, as well as the second cluster and PDZ7, are separated by largedomains of...
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Báo cáo khoa học: Investigations into the ability of an oblique a-helical template to provide the basis for design of an antimicrobial anionic amphiphilic peptide pot
... 389–395.43 Sospedra P, Espina M, Gomara MJ, Alsina MA, HaroI & Mestres C (2001) Study at the air ⁄ water interface of a hepatitis A N-acetylated and C-amidated syntheticpeptide (AcVP3 (110–121)-NH2). ... peptide characteristics and the com-position of its target membrane are important determinants of its efficacy of action.Abbreviations a- AMP, a- helical antimicrobial peptide; AP1, GEQGALAQFGEWL; ... membrane-interactive a- AMP.These data also suggest that the antimicrobial activity of AP1 depends on both the structural characteristics of its tilted peptide architecture and the lipid packingof...
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Báo cáo khoa học: Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt
... FEBSto an allosteric interaction between PAS and CAS[8,21,25], to a lower efficiency of the alternative routecompared to the main entrance, or to a partial overlap of the side chain of propidium and ... involving the hydroxyl of Tyr442 areindicated by a yellow dash. A BFig. 2. View of the back channel from the active site gorge of wild-type DmAChE (A) and Trp83Ala mutant (B). The protein databankcode ... by smaller side chains did not affect deacetyla-tion parameters k3 and a because the amino acid atposition 83 is too far away from the activated watermolecule during deacetylation. As expected,...
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Tài liệu Báo cáo khoa học: Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulfide bond formation and their contribution to venom complexity pdf
... (1994)Structural interaction of natural and synthetic inhibitorswith the venom metalloproteinase atrolysin C (form d).Proc Natl Acad Sci USA 91, 8447–8451.20 Kumasaka T, Yamamoto M, Moriyama H, Tanaka ... FEBS24 Watanabe L, Shannon JD, Valente RH, Rucavado A, Alape-Giron A, Kamiguti AS, Theakston RD, Fox JW,Gutierrez JM & Arni RK (2003) Amino acid sequence and crystal structure of BaP1, a metalloproteinase ... Golgi apparatus, and finally transported via secretory granules to the lumen of the venom gland [10]. On the basis of cDNA studies,all venom proteins have a signal sequence that probablytargets the...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... CTGACCTtcTGGCATAACACCGGtGAAATCAgeIW32F CCTGGTtcCATAACACCGGtGAAATCAgeIW240F GGTGCTgAGCTCAAGTGTGACTTtcGTCTACSacIW402F CCGGTGGTcGAcTTTGGTTtcACGCCCSalIE273Q ACGTACTGCTgTCCGGAAAGtACtCCATGGCCGCScaID353N ... ACGTACTGCTgTCCGGAAAGtACtCCATGGCCGCScaID353N TCCAATCCGTtAGTCTGgCCaTAGAACGCGCMscIE356Q GGAGAATGGTgCCAGGGTACATTTcCAATCCGTCAKpnID372N AATACATCTTaAGGCCGTCGTtGTCGGTGTGG A IID373N AATACATCTTaAGGCCGTtGCGTCGGTG A IIÓ FEBS ... rface between N-terminal and b-he licaldomains participate in binding of the polysaccharide,pullulan.Comparison of the active sites of the model of IPU and Dex4 9A The active site structures of...
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Tài liệu Báo cáo khoa học: "Insights into Non-projectivity in Hindi" docx
... us__lekhakiiy__asmitaa__ko ham sagarv prakaashak__ke−saamane rakhakar baat__karate_ _the b) a) _ROOT_ isake__baad vah jamaan__shaah aur−phir 1795__meM shaah__shujaa ko milaa this after it Jaman Shah and then ... then socialism was its solid baseb)_ROOT_ gorkii yadi is__naye__saahity__ke__srishtikartaa the to samaajavaad isakaa Thos aadhaar thaa a) _ROOT_ yadi rupayoM kii zaruurat thii to mujh ko bataanaa__chahiye__thaaif ... iden-tifying appropriate karakas. The dependency tagset in the annotationscheme has 28 relations in it. These includesix basic karaka relations (adhikarana [location],apaadaan [source], sampradaan [recipient],...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... 5¢-GCCCACACCAATACCTATGCG-3¢) and the 3¢ complementary primer 5¢-ATTAATGGCATGCTTTACCCGT-3¢.UsingthePCRproducts of agmatinase with the GfiAandCfiT substi-tutions in the coding and noncoding strands, ... Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and D153N mutant forms of E. coli agmatinase. Averagedistances ... pasted into the structural alignment. The alignment was then corrected byhand. Since the sequences of the two arginases and agmatinase greately differ in the N terminal region, the agmatinase...
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