Báo cáo khoa học: Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7 ) from zebrafish (Danio rerio) potx

... in the mass spectrometer from the facile loss of CO2(e.g. from either of the two c-carboxyglutamic acidresidues or other acidic groups) rather than from syntheticby-products based on the ... hift of the C3-carbon of the GalNAc (77. 4) strongly supported thiscarbon as the interglycosidic linkage carbon. Together thesedata suggested that the interglycosidic linkage betweenGalNAc and ... compared with the calculated monoisotopic[M-2CO2-H]–mass of 1840.42 Da). The mass shift of  4Da (MR ) M) confirms the formation of the twodisulfide bridges.However, comparison of the retention...

... H 8.5 (h), p H 7.5(n), pH 6.5 (e), and pH 5.5 (s) in PAT2 expressing oocytes. (C)Apparent Kmvalues of glycine (h )and L-alanine (s) – depict ed from (A) and (B) – as a function of the extracellular ... (s), pH 7.8 (j), and p H 7.5 (d). (B) PAT2 m ediated currents a s a function of the extracellular protonconcentration at various membrane p otentials: 0 mV (j), )2 0 mV ( m), )4 0 mV ( r), )6 0 ... as proposed for most of the other electrogenic symporters – the return of the unloadedtransporter to the outside of the membrane [17].In summary, we show that the mPAT2 protein whenexpressed...

... in the expression of ferrochelatase, which plays a role in the iron-removal reaction of exogenous heme and the change in position of the heme-moiety of hemopro-teins. The protoporphyrin ring of ... ferrochelatase in the outer membrane has a molecular mass similar to that of the enzyme in the inner membrane, the movement of the enzyme to the outer membrane may occur after the cleavage of the pre-sequence, ... grants from the Ministry of Education, Science, Sports and Culture of Japan.References1 Taketani S (199 4) Molecular and genetic characteriza-tion of ferrochelatase. In Regulation of Heme Protein Synthesis...

... domains and Table 1. Kinetic parameters for wild-type and mutant FlgCK constructs. Values represent mean ± 1 SD (n = 3). Construct KS(ADP)(lM) KM(ADP)(lM) KS(PCr)(mM) KM(PCr)(mM) KS⁄ ... nuclear gene families, eachtargeted to and localized in specific intracellularcompartments – cytoplasmic (CyCK), mitochondrial(MtCK) and flagellar (FlgCK). Two of these isoforms,CyCK and MtCK, ... are reported for the trimeric molecule.ConstructVmax(lmolÆmin )1 Æmg protein )1 ) kcat(s )1 ) kcat⁄ KM(PCr)(s )1 ÆmM )1 ) Wild-type 328 ± 16.0 715 ± 34.9 330D1SD2D3 270 ± 9.1ab586...

... the dorsal surface and into the gut of the amphibian [1–4]. A number of different types of bioactive peptides have been identified from the glandularskin secretions of Australian anurans of the ... 3 (anticancer activities), the numbers 5 and 6 refer to 10 )5 and 10 )6 M, respectively. Ten of the peptides failed the first stage of the anticancer testingprogram and are specified as ÔinactiveÕ: ... reflect the poorly definednature of the first turn of the a-helix due to the lack of hydrogen bonds to their NH protons.Comparison of the observed NH chemical shifts of A4K14-citropin 1.1 with the...

... towards the 3¢ end of the topstrand and the 5¢ end of the bottom strand (Fig. 4B).This agrees with the presence of a larger residue thatmakes stable contacts with this region of DNA. The interaction ... standard (S) to calculate the position of the footprint. Letters to the right of eachpanel indicate the DNA sequence (5¢ end in the upper part) of the corresponding strand in this region. In the lower ... nucleotides from the strandcarrying the sequence TGTGACA (hereafter named the top strand). The protected area includes GACA and two adjacent nucleotides (GA) towards the 3¢ end(Fig. 2A). On the bottom...

... (located at the C-terminus of the a2-helix) and Ala112 (located at the C-terminus of the b7-strand connected with the base of the b5 ⁄ b6 loop by hydrogen bonds). The conforma-tional change of Ala112 ... be PS and >90% of PS islocated at the inner leaflet [17–19]. The ratio of the PHdomain partitioned into supernatant and pellet wasdetermined from the densities of the SDS ⁄ PAGE bands and ... induced by the hydrophobic interaction between the a2-helix and the membrane interface were lost in the structure of the PHdomain at the surface of the PC⁄ PS⁄ PIP2vesicle, and consequently the structure...

... evolution of the spectra when the indicated number of Cd(II) was added. (B) CD spectrum of the acidification of the final solution of the Cd(II) titration of Zn–ckMT, the arrow shows the evolution from ... solutions of the Cd(II) titrations of Zn4–ackMT and Zn7–ckMT wereacidified from pH 7 to pH 4 (Fig. 3B), which caused the disappearance of the 250 nm absorptions and the preservation of all the other ... corresponding to the titrations of (A) Zn4–ackMT (B) Zn3–bckMT, and (C) Zn7–ckMTwith Cu(I) at pH 7. The arrows show the evolution of the spectra when the indicated number of Cu(I) equiv-alents...